PE/Cyanine7 Rabbit anti-Human CD2 Monoclonal Antibody for FC - P06729

Core Product Specifications and Parameters

Biological Background: CD2 Function and Localization

• CD2 is a T-cell surface antigen also known as LFA-2, SRBC, and T11, belonging to the immunoglobulin superfamily.
• It functions as a cell adhesion molecule by binding to CD58 (LFA-3) and CD48/BCM1, mediating T-cell interactions with other cells.
• The cytoplasmic domain of CD2 is involved in T-cell triggering and signaling transduction upon engagement.
• CD2 is localized to the cell membrane as a single-pass type I membrane protein with a signal peptide and transmembrane helix.
• It contains immunoglobulin-like domains and is known to form disulfide bonds, contributing to its structural stability.
• The protein is expressed in natural killer cells (at protein level) and is crucial for immune surveillance.
• Post-translational modifications include glycosylation, as CD2 is classified as a glycoprotein.
• The molecular weight of the mature protein is approximately 39.4 kDa (calculated from sequence).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 25–209 of human CD2, encompassing the extracellular domain, which may favor recognition of native surface-expressed CD2 in flow cytometry.
• For flow cytometry, consider titrating the antibody to determine optimal staining concentration and include appropriate isotype and negative controls.
• Since CD2 is expressed on T cells and NK cells, verify reactivity in relevant cell populations; note that receptor internalization may occur upon binding and can affect staining intensity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated sourcing partner for IVD raw materials, supporting T-cell biology and immuno-oncology research with high-quality reagents like this CD2 antibody. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and efficient sourcing for rare targets to accelerate your diagnostic development from concept to clinic.