PE/Cyanine7 Mouse anti-Human CD45RO mAb - P08575

Core Product Specifications and Parameters

Biological Background: CD45RO Function and Localization

• CD45 (PTPRC) is a receptor-type tyrosine-protein phosphatase essential for T-cell activation through the antigen receptor. It acts as a positive regulator of T-cell coactivation upon binding to DPP4. Related references: PMID:35767951
• The protein contains two PTPase domains; the first has enzymatic activity while the second modulates substrate specificity of the first. Upon T-cell activation, CD45 dephosphorylates signaling molecules such as SKAP1, FYN, and LYN.
• CD45RO is an isoform generated by alternative splicing, lacking certain extracellular domains compared to other isoforms like CD45RA. Multiple isoforms show distinct expression patterns in thymocytes.
• It is located at the cell membrane, membrane rafts, and immunological synapse, positioning it for crucial roles in immune cell signaling.
• CD45 interacts with CLEC10A on immature dendritic cells at the T-cell contact site, modulating T-cell activation threshold to limit autoreactivity.
• Post-translational modifications include heavy glycosylation and phosphorylation, contributing to its large molecular weight and function.
• (Microbial infection) CD45 also acts as a receptor for human cytomegalovirus protein UL11, impairing T-cell proliferation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from human CD45RO, suggesting specificity for the CD45RO isoform. For flow cytometry, consider titrating the antibody to determine optimal staining concentration for your cell population.
• Since this antibody is PE/Cyanine7-conjugated, use appropriate laser and filter settings (e.g., blue laser, 780/60 nm filter). Ensure compensation controls are included.
• Validate antibody performance in the relevant sample system (e.g., human PBMCs) by including appropriate isotype controls and positive/negative cell populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner, offering validated antibodies, bulk ancillary reagents, and rare target materials. This CD45RO antibody supports immunological research such as T-cell phenotyping and activation studies. We provide comprehensive supply solutions from concept to clinic.