PE/Cyanine7 Mouse anti-Human CD11b Monoclonal Antibody for Flow Cytometry - P11215

Core Product Specifications and Parameters

Biological Background: Integrin alpha-M (CD11b) Function and Localization

• Integrin alpha-M (ITGAM/CD11b) is also known as CD11 antigen-like family member B, CR-3 alpha chain, MAC-1 subunit alpha, MO1, and neutrophil adherence receptor.
• ITGAM combines with ITGB2 (CD18) to form the Mac-1 integrin, which mediates adhesive interactions of monocytes, macrophages, and granulocytes and facilitates the uptake of complement-coated particles and pathogens. Related references: PMID:20008295 PMID:9558116
• Mac-1 functions as the receptor for the iC3b fragment of complement component C3 and also serves as a receptor for fibrinogen and factor X.
• ITGAM plays a role in the regulation of neutrophil migration. Related references: PMID:28807980
• In association with ITGB2, ITGAM is required for CD177-PRTN3-mediated activation of TNF-primed neutrophils. Related references: PMID:21193407
• The integrin acts as a receptor for ICAM1, promoting adhesion between stimulated neutrophils and endothelial cells. Related references: PMID:1980124
• CD11b is predominantly expressed on monocytes and granulocytes, including neutrophils. Related references: PMID:1346576 PMID:21193407
• The protein localizes to the cell membrane and membrane rafts.

Experimental Guidance and Technical Tips

• This mouse monoclonal antibody, conjugated to PE/Cyanine7, targets a synthetic peptide derived from human CD11b and is validated for flow cytometry (FC). Titrate the antibody using human peripheral blood mononuclear cells (PBMCs) or whole blood samples to determine the optimal staining concentration.
• Include appropriate isotype and compensation controls to account for background and spectral overlap. Because CD11b expression can be modulated by cell activation, standardize sample preparation protocols and consider co-staining with activation markers if needed.
• Verify antibody binding specificity in your cell system by comparing with known CD11b-positive (monocytes, granulocytes) and -negative controls, or use blocking peptides/knockdown approaches where applicable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw material sourcing by providing validated antibody pairs, monoclonal and polyclonal antibodies, bulk reagents, and technical support. With expertise in immunology and infectious disease targets, we connect diagnostic manufacturers, labs, and research institutes to the critical raw materials they need. Whether you require CD11b antibodies for flow cytometry diagnostics or other rare target reagents, CamelBio offers one-stop access to quality raw materials and customized services to accelerate your project from concept to clinic.