PE/Cyanine7 Anti-Mouse Ly-6A/E (Sca-1) Monoclonal Antibody for Flow Cytometry - P05533

Core Product Specifications and Parameters

Biological Background: Lymphocyte antigen 6A-2/6E-1 (Sca-1) Function and Localization

• Lymphocyte antigen 6A-2/6E-1, commonly known as stem cell antigen 1 (Sca-1) or T-cell-activating protein (TAP), is encoded by the Ly6a gene in mice and belongs to the Ly-6/uPAR superfamily of glycosylphosphatidylinositol (GPI)-anchored proteins.
• Sca-1 is a well-established marker of murine hematopoietic stem cells and progenitor cells, and is widely expressed across multiple tissues.
• The primary function of Sca-1 is linked to T-cell activation, where it can modulate signaling upon engagement, contributing to immune response regulation.
• Sca-1 is anchored to the outer leaflet of the plasma membrane via a GPI moiety, with no transmembrane or cytoplasmic domain, relying on lipid raft association for signal transduction.
• As a GPI-anchored glycoprotein, Sca-1 undergoes post-translational modifications including N-linked glycosylation and the addition of the GPI anchor, which are critical for its membrane localization and function.
• The protein contains conserved disulfide bonds that stabilize its three-fingered fold characteristic of the Ly-6 family, facilitating protein-protein interactions.
• The observed molecular weight of Sca-1 often exceeds its calculated mass (14.4 kDa) due to extensive glycosylation, with typical detection around 115 kDa in SDS-PAGE under reducing conditions.

Experimental Guidance and Technical Tips

• This antibody was generated using a recombinant immunogen spanning the extracellular domain (amino acids 27-111) of mouse Sca-1, targeting the native conformation for superior recognition in flow cytometry.
• For flow cytometry applications, consider titrating the antibody to determine the optimal working concentration, and include appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding.
• Note that the PE/Cyanine7 conjugate is sensitive to light and freeze-thaw cycles; always protect from light and avoid repeated freezing.
• Validate the antibody specificity in the relevant mouse strain or cell line, as allelic variations (e.g., Ly-6A.2 vs Ly-6E.1) exist; the immunogen corresponds to the Ly-6A.2 allele.
• Sca-1 expression can be upregulated upon activation in T cells; time-course experiments may be necessary to capture dynamic expression changes.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated partner for IVD raw material procurement, CamelBio streamlines access to high-quality antibodies like this Sca-1 monoclonal antibody, supporting stem cell research, immunophenotyping, and T-cell activation studies. Our platform offers validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary supplies, and rare-target sourcing to meet the demands of diagnostic manufacturers, laboratories, and research institutes. From concept to clinic, CamelBio provides one-stop technical and supply solutions to accelerate your development pipelines.