PE/Cyanine7 Anti-Mouse CD3e Monoclonal Antibody for Flow Cytometry - P22646

Core Product Specifications and Parameters

Biological Background: CD3e Function and Localization

• CD3e is the epsilon chain of the T-cell surface glycoprotein CD3, also known as T3e or CD3epsilon, forming the TCR–CD3 complex.
• It plays an essential role in adaptive immune response by transducing TCR-mediated signals upon antigen recognition. Related references: PMID:9843989 PMID:10952314 PMID:19956738
• The cytoplasmic domain of CD3e contains immunoreceptor tyrosine-based activation motifs (ITAMs) that are phosphorylated by Src family kinases LCK and FYN, leading to ZAP70 recruitment and activation. Related references: PMID:10952314 PMID:19956738 PMID:24899501
• Beyond signaling, CD3e is critical for proper T-cell development. Related references: PMID:10952314 PMID:19956738 PMID:24899501
• Localized at the cell membrane, it functions as a single-pass type I membrane protein.
• CD3e also mediates internalization and down-regulation of TCR–CD3 complexes via endocytosis motifs, and serves as a receptor for ITPRIPL1 to inhibit T-cell activation (By similarity).
• Structurally, it contains an immunoglobulin domain, a disulfide bond, and a transmembrane helix.

Experimental Guidance and Technical Tips

• Since the immunogen is recombinant protein, the antibody may recognize linear epitopes; consider this when designing experiments involving denaturing conditions.
• For flow cytometry, validate optimal dilution and staining conditions in your specific sample system, including appropriate isotype controls and Fc receptor blocking.
• The antibody is conjugated to PE/Cyanine7; ensure that the fluorophore is compatible with your instrument's laser and filter configuration, and avoid prolonged light exposure during staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to high-quality IVD raw materials, facilitating seamless transition from concept to clinic. This anti-CD3e monoclonal antibody, along with a broad portfolio of immune-related reagents, supports your immunophenotyping and T-cell research needs. Leverage our expertise in sourcing validated antibody pairs, optimized monoclonals, and bulk ancillary reagents to accelerate your assay development.