PE/Cyanine7 Anti-Mouse CD161c/NK1.1 Monoclonal Antibody for Flow Cytometry - P27814

Core Product Specifications and Parameters

Biological Background: CD161c/NK1.1 (NKR-P1C) Function and Localization

• CD161c/NK1.1 (gene Klrb1c) is a killer cell lectin-like receptor subfamily B member 1C, also known as NKR-P1C, Lymphocyte antigen 55c, or CD161 antigen-like family member C.
• The receptor plays a stimulatory role in natural killer (NK) cell-mediated cytotoxicity, with cross-linking leading to Ca²⁺ mobilization and interferon-gamma (IFN-γ) production.
• It is a transmembrane glycoprotein located on the plasma membrane, consistent with its function in extracellular ligand recognition and signal transduction.
• Expression is predominantly restricted to natural killer cells, as indicated by tissue specificity data.
• The protein contains a C-type lectin domain and features disulfide bonds, and alternative splicing generates multiple isoforms.
• Keywords: Alternative splicing, Disulfide bond, Glycoprotein, Lectin, Membrane, Signal-anchor, Transmembrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 67-223 of mouse CD161c/NK1.1, which encompasses the extracellular lectin-like domain; this region is likely accessible on the cell surface for flow cytometry applications.
• When designing flow cytometry panels, consider titrating the antibody to determine the optimal concentration for your specific NK cell subset and experimental conditions.
• Use appropriate isotype controls and Fc receptor blocking reagents to minimize non-specific binding, especially when working with Fc receptor-expressing cells.
• For functional studies, note that cross-linking of the receptor can induce activation signals; avoid unintended cross-linking by using monovalent Fab fragments if activation is not desired.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This PE/Cyanine7 anti-mouse CD161c/NK1.1 monoclonal antibody supports NK cell immunophenotyping and functional research. Beyond this product, we offer tailored sourcing solutions for rare targets, supporting your projects from concept to clinic.