PE/Cyanine7 anti-Mouse CD11c Monoclonal Antibody for FC - Q9QXH4

Core Product Specifications and Parameters

Biological Background: CD11c Function and Localization

• CD11c, also known as Integrin alpha-X, is a member of the integrin family and the CD11 antigen-like family. It is encoded by the Itgax gene in mice.
• Integrin alpha-X pairs with integrin beta-2 to form the leukocyte adhesion receptor p150,95. This heterodimer serves as a receptor for fibrinogen, recognizing the G-P-R sequence.
• The p150,95 integrin mediates cell-cell interactions during inflammatory responses, playing a crucial role in monocyte adhesion and chemotaxis.
• Additionally, Integrin alpha-X functions as a receptor for the erythrocyte-specific adhesion molecule ICAM4, facilitating erythrophagocytosis.
• CD11c is predominantly localized to the cell membrane as a single-pass type I transmembrane protein.
• Consistent with its role in adhesion and signaling, Integrin alpha-X is a glycoprotein containing disulfide bonds and calcium/magnesium-binding domains, characteristic of integrin receptors.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to PE/Cyanine7, making it suitable for use in flow cytometry (FC) without the need for a secondary antibody.
• Based on the recombinant protein immunogen, the antibody may recognize both native and denatured forms of CD11c, but validation in the relevant sample system is recommended.
• Titration experiments should be considered to determine the optimal antibody concentration for specific cell types and flow cytometry protocols.
• Since CD11c is expressed on leukocytes, particularly myeloid cells, use appropriate mouse leukocyte samples (e.g., spleen, blood, bone marrow) for testing.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio offers reliable access to high-quality IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like anti-CD11c, supporting immunology and inflammation research.
• Beyond antibodies, CamelBio provides bulk ancillary reagents and rare-target sourcing capabilities to streamline your diagnostic or laboratory workflows from concept to clinic.