PE/Cyanine7 Anti-Human CD27 Monoclonal Antibody for Flow Cytometry - P26842

Core Product Specifications and Parameters

Biological Background: CD27 Function and Localization

• CD27 (also known as TNFRSF7, T14, Tp55, S152) is a member of the tumor necrosis factor receptor superfamily, functioning as a costimulatory immune-checkpoint receptor.
• It is expressed on the surface of T-cells, NK-cells, and B-cells, and binds to its ligand CD70 (CD27L) on B-cells to mediate antigen-specific T-cell activation and expansion (PubMed:28011863). Related references: PMID:28011863
• CD70 ligation activates the TRAF2-PTPN6 axis, inhibiting LCK phosphorylation to promote T-cell memory phenotypic and transcriptional adaptations (PubMed:38354704). Related references: PMID:38354704
• In early progenitor cells, CD70 activation provides a negative feedback signal to leukocyte differentiation, thereby modulating hematopoiesis.
• CD27 negatively regulates Th2 lymphocyte function in adipose tissue.
• The protein localizes to the cell membrane and contains a signal peptide, transmembrane helix, and extracellular domains with repeat structures.
• Post-translational modifications include glycosylation and phosphorylation; it also features disulfide bonds.
• Key associated biological processes: apoptosis, immune response, and hematopoiesis regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 21-192) of human CD27, which ensures recognition of the native receptor in flow cytometry.
• PE/Cyanine7 is a bright fluorochrome suitable for detecting low-abundance surface markers; consider using appropriate compensation controls when combining with other PE- or Cyanine7-conjugated antibodies.
• For flow cytometry applications, validate the antibody in the relevant sample system (e.g., human PBMCs) with proper Fc blocking and viability staining.
• Due to the target's role in T-cell activation, analyze expression on different T-cell subsets (naïve, memory, effector) to assess differentiation status.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we understand the critical need for reliable raw materials in diagnostic development. As your one-stop IVD raw-material partner, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents to support your immunology and T-cell research workflows. Our sourcing capabilities extend to rare targets like CD27, ensuring you have continuous access to high-quality materials from concept to clinic.