PE/Cyanine5 Rabbit anti-Mouse CD90.2 mAb for Flow Cytometry - P01831

Core Product Specifications and Parameters

Biological Background: CD90.2 Function and Localization

• Thy-1 membrane glycoprotein (CD90.2) is a cell surface protein anchored to the membrane via a glycosylphosphatidylinositol (GPI) anchor.
• It belongs to the immunoglobulin superfamily and contains a single immunoglobulin-like domain, facilitating interactions with other cell surface or matrix proteins.
• CD90.2 is expressed on various cell types, including thymocytes, T lymphocytes, neurons, and mesenchymal stem cells, where it may modulate adhesion and signaling.
• Its core function involves mediating cell-cell or cell-ligand interactions, with a proposed role in synaptogenesis and neuronal development in the brain (UniProt).
• Post-translational modifications include signal peptide cleavage, N-linked glycosylation, disulfide bond formation, and GPI-anchor attachment, which are critical for its membrane localization and stability.
• As a GPI-anchored protein, CD90.2 is enriched in lipid rafts and may participate in signal transduction upon cross-linking.
• The mouse CD90 exists in two allelic forms, CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2), distinguished by a single amino acid polymorphism; this antibody specifically recognizes CD90.2.

Experimental Guidance and Technical Tips

• This PE/Cyanine5-conjugated monoclonal antibody is designed for flow cytometry application; verify performance in your specific cell system.
• Titrate the antibody to determine optimal staining concentration for your target cell population, using appropriate isotype and fluorescence-minus-one controls.
• Since CD90.2 is GPI-anchored, consider using gentle cell dissociation reagents (e.g., non-enzymatic methods) to preserve surface antigen integrity.
• Validate staining on known positive cells (e.g., mouse thymocytes or T cell lines) and negative controls to confirm specificity.
• Protect the conjugate from prolonged light exposure to maintain fluorophore stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials and technical support to accelerate your diagnostic development. This PE/Cyanine5 anti-mouse CD90.2 antibody supports immune cell profiling and neuroscience research applications. Beyond this product, CamelBio can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target raw materials—all through a single, efficient procurement channel.