PE/Cyanine5 Rabbit anti-Mouse CD5 Monoclonal Antibody for Flow Cytometry - P13379

Core Product Specifications and Parameters

Biological Background: CD5 Function and Localization

• T-cell surface glycoprotein CD5, also known as lymphocyte antigen 1 (Ly-1/Lyt-1), is a lymphoid-specific receptor expressed by all T-cells and the B1a subset of B-cells.
• Core function involves regulation of TCR and BCR signaling, controlling thymocyte selection, effector differentiation, and immune tolerance (PubMed:35720357). Related references: PMID:35720357
• Mediates T-dependent B-cell activation and homeostasis through interaction with ligands CD5L and CD72 on B-cells (PubMed:24950378, PubMed:9064341, PubMed:9723705). Related references: PMID:24950378, PMID:9064341, PMID:9723705
• Negative regulation of TCR signaling involves recruitment of LCK, CD3ζ, PI3K, and CBL; co-engagement with CD3 enhances CBL-mediated VAV1 degradation (PubMed:11157848). Related references: PMID:11157848
• In B-cells, modulates ERK1/2 activation via TRPC1-dependent Ca2+ entry, leading to IL-10 production (By similarity).
• Localized at the cell membrane; the protein undergoes glycosylation, phosphorylation, and disulfide bond formation.

Experimental Guidance and Technical Tips

• This PE/Cyanine5-conjugated antibody allows direct flow cytometry detection of cell surface CD5 on viable mouse cells. Prepare single-cell suspensions from lymphoid organs and block Fc receptors before staining.
• Titrate the antibody to determine the optimal concentration for your specific model; include an appropriate isotype control and viability dye.
• CD5 is expressed on all T-cells and B1a B-cells; use in combination with lineage markers for precise immunophenotyping.
• Validate the assay in your laboratory using relevant positive (e.g., splenocytes) and negative (e.g., non-lymphoid cells) controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, providing a streamlined bridge from concept to clinic. This PE/Cyanine5 anti-mouse CD5 monoclonal antibody supports T-cell and B1a cell immunophenotyping applications. From validated antibody pairs and bulk ancillaries to custom conjugation and rare target reagents, our comprehensive portfolio helps diagnostic manufacturers and researchers accelerate their workflows with confidence.