PE/Cyanine5 Rabbit Anti-Mouse CD45 Monoclonal Antibody for Flow Cytometry - P06800

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45, also known as leukocyte common antigen (L-CA), lymphocyte antigen 5 (Ly-5), and T200, is a receptor-type tyrosine-protein phosphatase C encoded by the Ptprc gene. Multiple isoforms are generated via alternative splicing, including CD45R and B220, with molecular weights ranging from 130 to 144 kDa.
• It functions as a critical regulator of T-cell activation through the antigen receptor, acting as a positive coactivator upon binding to DPP4. The first of its two intracellular phosphatase domains has enzymatic activity, while the second modulates substrate specificity.
• CD45 dephosphorylates key signaling molecules such as SKAP1, FYN, and LYN to modulate their activity. It also interacts with CLEC10A on dendritic cells, linking glycosylation sensing to T-cell activation thresholds.
• The protein localizes to the cell membrane, membrane rafts, and immunological synapse, positioning it as a key mediator of signaling at T-cell–antigen-presenting cell contacts.
• CD45 is heavily modified by glycosylation and phosphorylation, as reflected by its UniProt keywords: glycoprotein, phosphoprotein. It spans the membrane via a single transmembrane helix.
• Predominantly expressed on all leukocytes (hence "common antigen"), CD45 is essential for lymphocyte development and function. Its molecular weight is approximately 145 kDa, with isoforms varying due to extracellular domain splicing.

Experimental Guidance and Technical Tips

• For flow cytometry, consider titrating the antibody to determine the optimal concentration for your cell population and staining conditions.
• Include appropriate isotype controls and compensation controls when setting up multicolor panels.
• The immunogen corresponds to the extracellular domain (amino acids 26–566), suggesting the antibody likely recognizes surface epitopes; pre-blocking with Fc receptor blockers may reduce non-specific binding.
• Validate performance in the relevant mouse leukocyte subset, as expression levels can vary among lymphocyte populations.

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