PE/Cyanine5 Rabbit anti-Mouse CD11b Monoclonal Antibody for Flow Cytometry - P05555

Core Product Specifications and Parameters

Biological Background: CD11b Function and Localization

• CD11b, also known as integrin alpha-M or CR3 alpha chain, is encoded by the Itgam gene and pairs with integrin beta-2 (CD18) to form the complement receptor 3 (CR3) heterodimer.
• ITGAM/ITGB2 mediates adhesive interactions of monocytes, macrophages, and granulocytes, and facilitates uptake of complement-coated particles and pathogens via the iC3b fragment of complement component 3.
• The integrin also acts as a receptor for fibrinogen, factor X, and ICAM1, enabling adhesion between stimulated neutrophils and endothelial cells.
• CD11b localizes to the cell membrane and membrane rafts, consistent with its role as a transmembrane receptor.
• In microglia, CD11b collaborates with TYROBP/DAP12 to regulate superoxide ion production, which promotes neuronal apoptosis during brain development. Related references: PMID:18685038
• Predominantly expressed in monocytes and granulocytes, with additional presence in subsets of peritoneal mast cells and microglia.
• Features a signal peptide, a large extracellular region with multiple repeats, a transmembrane helix, and key post‑translational modifications including glycosylation and disulfide bonds.
• Alternative splicing generates multiple isoforms, potentially contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular region (aa 143–337) of mouse CD11b, suggesting the antibody may recognize an epitope within this domain; verify reactivity with your target cell population.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and staining protocol.
• Include appropriate isotype controls and, if multiplexing, compensation controls to ensure accurate fluorescence signal interpretation.
• Consider potential cross-reactivity with other integrin alpha chains if epitope conservation exists; validate in the relevant mouse sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies CD11b antibodies and supportive IVD raw materials to diagnostic developers and research laboratories worldwide. Beyond this PE/Cyanine5-conjugated monoclonal antibody, we offer validated antibody pairs, bulk ancillary reagents, and secure sourcing for rare targets in myeloid cell and immunology research. Our integrated procurement network helps maintain assay consistency and supply continuity from concept to clinic.