PE/Cyanine5 Mouse anti-Human CD3 Monoclonal Antibody for Flow Cytometry - P07766

Core Product Specifications and Parameters

Biological Background: CD3 epsilon Function and Localization

• CD3E encodes the CD3 epsilon chain, a core subunit of the T-cell receptor (TCR)-CD3 complex, which is essential for adaptive immunity by transducing activation signals after antigen recognition. Related references: PMID:15294938 PMID:15546002 PMID:2470098
• Intracellular immunoreceptor tyrosine-based activation motifs (ITAMs) in CD3E are phosphorylated by LCK and FYN kinases upon TCR engagement, creating docking sites for ZAP70 and initiating downstream signaling cascades. Related references: PMID:2470098 PMID:40592325
• CD3E contributes to correct T-cell development and thymocyte selection, ensuring proper immune repertoire formation.
• The cytoplasmic domain of CD3E contains endocytosis motifs that mediate TCR-CD3 internalization and down-regulation, modulating T-cell responsiveness after stimulation. Related references: PMID:10384095 PMID:26507128
• As a receptor for ITPRIPL1, CD3E can inhibit T-cell activation by recruiting NCK1, which prevents ZAP70 binding and blocks ERK-NFkB signaling and calcium influx. Related references: PMID:12110186 PMID:38614099
• CD3E is a single-pass type I membrane protein with an extracellular immunoglobulin-like domain, a transmembrane helix, and a cytoplasmic tail; it localizes exclusively to the plasma membrane.
• Post-translational modifications include ITAM phosphorylation and a conserved disulfide bond that stabilizes its extracellular structure.

Experimental Guidance and Technical Tips

• This antibody, raised against recombinant human CD3 protein, is validated for flow cytometry; for other applications, consider running pilot experiments to confirm reactivity in your sample system.
• When setting up multicolor flow cytometry panels, include appropriate compensation controls and titrate the antibody to determine the optimal staining concentration.
• As a tandem dye conjugate, PE/Cyanine5 is sensitive to light and fixatives; minimize light exposure, use ice-cold staining buffers, and avoid prolonged fixation to preserve signal intensity.
• For intracellular staining or phospho‑CD3 detection, validate permeabilization conditions, as harsh treatments may alter epitope accessibility or dye stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw materials, offering this PE/Cyanine5 anti-human CD3 monoclonal antibody alongside a wide portfolio of validated antibody pairs, optimized bulk reagents, and ancillary products for immune monitoring assays. Whether you need rare-target sourcing or consistent lot-to-lot performance, our supply chain supports every stage of diagnostic development, helping you move from concept to clinic efficiently.