Pea3 / ETV4 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P43268

Core Product Specifications and Parameters

Biological Background: ETV4 Function and Localization

• ETV4, also known as PEA3 or E1A-F, is a member of the ETS transcription factor family and is encoded by the ETV4 gene on chromosome 17 in humans.
• It functions as a transcriptional activator, binding to the core DNA motif 5'-[AC]GGA[AT]GT-3' to regulate gene expression (PubMed:19307308, PubMed:31552090). Related references: PMID:19307308, PMID:31552090
• ETV4 is critical for neuronal development, regulating motor neuron positioning within the spinal cord and controlling terminal arborization of specific motor neuron axons in their target muscles (By similarity).
• The protein localizes exclusively to the nucleus, consistent with its role in transcriptional regulation.
• ETV4 undergoes alternative splicing and is subject to post-translational modifications including phosphorylation and ubiquitination (isopeptide bond), indicating regulatory control.
• In keratinocytes, ETV4 contributes to differentiation, and its expression may be relevant to skin biology and pathology (PubMed:31552090). Related references: PMID:31552090
• During adenovirus infection, ETV4 binds to the E1A enhancer and acts as a transcriptional activator, highlighting its role in viral gene regulation.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted band is approximately 54 kDa; consider including positive controls such as HeLa or 293T cell lysates and validate in the relevant sample system.
• In immunohistochemistry (IHC-P), antigen retrieval using citrate buffer (pH 6.0) or EDTA (pH 9.0) may be necessary; optimize based on tissue type and fixation.
• For immunofluorescence (IF/ICC), expect nuclear staining; use appropriate detergents for permeabilization and confirm specificity with a blocking peptide or knockdown control.
• As a polyclonal antibody, verify lot-to-lot consistency for critical assays and consider affinity purification if higher specificity is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials and technical support, from concept to clinic. The ETV4/Pea3 polyclonal antibody supports studies on transcription regulation, neurogenesis, and cancer, and CamelBio can assist in sourcing validated antibody pairs, bulk ancillary reagents, and rare-target raw materials for your assay development programs. Partner with CamelBio to accelerate your biomarker discovery and diagnostic kit production with reliable supply chain solutions.