PEA15 Rabbit Polyclonal Antibody for WB and ELISA - Q15121

Core Product Specifications and Parameters

Biological Background: PEA-15 Function and Localization

• PEA-15 (Astrocytic phosphoprotein PEA-15), also known as PED (Phosphoprotein enriched in diabetes), is a 15 kDa multifunctional adaptor protein encoded by the PEA15 gene on human chromosome 1. Alternative splicing produces isoforms, with isoform 1 being the canonical sequence.
• It functions as a key regulator of the ERK MAP kinase cascade by blocking Ras-mediated inhibition of integrin activation and by inhibiting RPS6KA3 activities through cytoplasmic retention (By similarity).
• PEA-15 suppresses both TNFRSF6- and TNFRSF1A-mediated extrinsic apoptosis by inhibiting CASP8 activation, highlighting its role in cell survival signaling.
• The protein controls glucose transport by regulating SLC2A1 (GLUT1) plasma membrane content and insulin-dependent trafficking of SLC2A4 (GLUT4), making it important for glucose homeostasis.
• Ubiquitously expressed, PEA-15 is most abundant in heart, brain, muscle, and adipose tissue, with elevated levels observed in type 2 diabetes tissues.
• Localized primarily in the cytoplasm, PEA-15 is a phosphoprotein subject to post-translational phosphorylation that modulates its activity and interactions.
• Keywords also include 3D-structure, direct protein sequencing, and proteomics identification, underscoring its structural characterization and role in signaling networks.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 130 amino acids of human PEA15, covering the death effector domain (DED) critical for apoptosis and signaling interactions. Consider this epitope region when designing orthogonal validation assays.
• For Western blot (WB), the predicted molecular weight is 15 kDa; ensure adequate separation and transfer conditions for small proteins. Validate band specificity using appropriate positive and negative controls in your sample system.
• This antibody cross-reacts with both human and mouse PEA15; if using mouse tissue, consider potential cross-reactivity with endogenous immunoglobulins in immunohistochemical applications and use appropriate secondary antibody controls.
• ELISA performance may depend on coating antigen format; consider using the recombinant immunogen or native protein from relevant cell lysates as capture target. Optimize blocking and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This anti-PEA15 rabbit polyclonal antibody fits into apoptosis, glucose metabolism, and diabetes research areas, and we can further supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing to streamline your assay development and production workflows. Let CamelBio be your bridge to reliable, high-quality immunoreagents.