PE Rat anti-Mouse Ly-6G/Ly-6C (Gr-1) Monoclonal Antibody for FC - P35461 / P0CW02

Core Product Specifications and Parameters

Biological Background: Ly-6G/Ly-6C Function and Localization

• Gr-1 is a granulocyte-specific antigen composed of two GPI-anchored proteins, Ly-6G and Ly-6C.
• Ly-6G (SWISS: P35461) is designated lymphocyte antigen 6G, also known as Ly-6G.1, and is a cell membrane protein anchored via glycosylphosphatidylinositol (GPI).
• The protein contains a signal peptide, intrachain disulfide bonds, and is glycosylated.
• Ly-6G is expressed specifically in bone marrow tissues.
• The mature Ly-6G protein has a calculated molecular weight of approximately 14 kDa.
• Ly-6C (SWISS: P0CW02) is a related Ly-6 family member; together with Ly-6G it forms the well-established Gr-1 marker for mouse granulocytes and monocytes in flow cytometry.

Experimental Guidance and Technical Tips

• The recombinant protein immunogen suggests that the antibody may recognize surface‑exposed epitopes; optimize staining on viable cells for flow cytometry.
• Validate performance in mouse bone marrow or peripheral blood leukocyte preparations, where target expression is expected.
• Include an appropriate isotype control and Fc receptor blocking step to minimize background in flow cytometry experiments.
• Titrate the antibody concentration to achieve optimal signal‑to‑noise ratios for your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing a comprehensive portfolio of IVD raw materials, including validated antibodies for granulocyte and monocyte marker detection. We offer monoclonal and polyclonal antibodies, bulk ancillary reagents, and dedicated sourcing for rare‑target raw materials—streamlining your workflow from concept to clinic.