PE Rat anti-Mouse Ly-6G mAb for FC - P35461

Core Product Specifications and Parameters

Biological Background: Lymphocyte antigen 6G Function and Localization

• Lymphocyte antigen 6G (Ly-6G), also designated Ly-6G.1, is encoded by the Ly6g gene in mouse.
• It is a cell membrane protein characterized by a glycosylphosphatidylinositol (GPI) anchor that tethers it to the outer leaflet of the plasma membrane.
• The protein contains an intramolecular disulfide bond and is classified as a glycoprotein, with a predicted molecular weight of approximately 14 kDa.
• Expression of Ly-6G is predominantly observed in bone marrow tissue, consistent with its detection in hematopoietic compartments.
• UniProt keywords for Ly-6G include Cell membrane, Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Membrane, Reference proteome, and Signal, highlighting its post-translational modifications and membrane association.

Experimental Guidance and Technical Tips

• This PE-conjugated monoclonal antibody is ready for direct flow cytometry (FC) without the need for a secondary reagent, streamlining single-step staining protocols.
• The immunogen is a synthetic peptide derived from mouse Ly-6G; thus, the antibody likely recognizes a linear epitope and may be suitable for detection under both native and denaturing conditions, though denaturing applications should be validated.
• For FC, consider using appropriate isotype controls and titrate the antibody on your target cell population (e.g., bone marrow-derived myeloid cells) to optimize signal-to-noise ratio.
• Cross-reactivity has been demonstrated for mouse; reactivity with other species has not been tested and should be assessed if required.

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CamelBio is a comprehensive sourcing partner for IVD raw materials, serving diagnostic manufacturers and researchers with validated antibodies, bulk ancillary reagents, and rare target sourcing. Our portfolio includes optimized monoclonal antibodies like PE Rat anti-Mouse Ly-6G, supporting immunology and flow cytometry workflows. From concept to clinic, we provide one-stop access to the reagents and technical support needed to accelerate your diagnostic development.