PE Rat anti-Mouse CD45R/B220 Monoclonal Antibody for Flow Cytometry - P06800 / P08575

Core Product Specifications and Parameters

Biological Background: CD45R/B220 Function and Localization

• CD45R/B220, encoded by the Ptprc gene, is a receptor-type tyrosine-protein phosphatase (PTPase) also known as Leukocyte Common Antigen (L-CA) and Lymphocyte Antigen 5 (Ly-5). It is predominantly expressed on hematopoietic cells, including B and T lymphocytes, and exists in multiple isoforms generated by alternative splicing.
• The protein serves as a critical regulator of T-cell receptor signaling by modulating the activity of Src family kinases such as LYN and FYN. It dephosphorylates LYN, influencing downstream signaling cascades essential for T-cell activation and coactivation upon binding to DPP4.
• At antigen-presenting cell (APC)–T cell immunological synapses, CD45 interacts with CLEC10A on immature dendritic cells, which recognizes Tn antigen-carrying CD45 on effector T cells. This interaction helps fine-tune T-cell activation thresholds and limit autoreactive responses (By similarity).
• CD45 contains two intracellular phosphatase domains: the first domain exhibits catalytic activity, while the second domain appears to regulate substrate specificity. The extracellular region is heavily glycosylated and contributes to ligand interactions and isoform-specific functions.
• The protein localizes primarily to the plasma membrane and is enriched in membrane rafts and immunological synapses, where it spatially organizes signaling complexes following antigen engagement.
• As a transmembrane glycoprotein and a member of the protein phosphatase family, CD45 is a well-established marker for immune cell lineages. The B220 isoform is specifically associated with B cells and is widely used to identify B cell populations in mouse models.
• Post-translational modifications, including phosphorylation and glycosylation, modulate CD45 enzymatic activity and cell-surface expression, thereby influencing immune cell signaling dynamics.
• Although primarily studied in lymphoid cells, CD45 also plays roles in other leukocyte subsets, contributing to integrated innate and adaptive immune responses.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from a conserved region of mouse/human CD45R/B220. The resulting antibody is expected to recognize mouse CD45R/B220; cross-reactivity with human orthologs should be experimentally validated if needed.
• This antibody is conjugated to PE (phycoerythrin), a bright fluorophore suitable for multicolor flow cytometry panels. Consider titrating the antibody to optimize signal-to-noise ratios and minimize non-specific binding in your specific cell system.
• For flow cytometry applications, use appropriate viability dyes and Fc receptor blocking reagents to reduce background staining. Analyze fresh or properly cryopreserved mouse lymphoid tissues (spleen, lymph nodes) to detect B220-positive B cell populations.
• Given the reported molecular weight range (130–144 kDa) reflecting alternative splicing and glycosylation, confirm expression and band size by western blotting if adapting this product for orthogonal detection methods, though the primary validated application is flow cytometry.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to high-quality IVD raw materials, including a curated portfolio of conjugated monoclonal antibodies for immune profiling. Our supply chain supports bulk procurement of validated antibody pairs, ancillary reagents, and rare-target raw materials, streamlining your development from assay design to clinical application. With this CD45R/B220 antibody, we offer a reliable tool for B cell phenotyping and immune status monitoring, aligned with rigorous quality standards for reproducible results.