PE Rat anti-Mouse CD3 Monoclonal Antibody for Flow Cytometry - P04235 / P22646

Core Product Specifications and Parameters

Biological Background: CD3 Function and Localization

• CD3 is a multi-chain complex composed of delta, epsilon, gamma, and zeta chains; this antibody reacts with the CD3 delta and epsilon chains (SWISS: P04235/P22646) that assemble with the T-cell receptor (TCR) to form the TCR-CD3 complex on T lymphocytes.
• The TCR-CD3 complex is essential for adaptive immunity, transducing signals from antigen-presenting cells upon TCR engagement via immunoreceptor tyrosine-based activation motifs (ITAMs) in the cytoplasmic domains of CD3 chains.
• CD3 delta (P04235) plays a critical role in thymocyte differentiation; in the absence of a functional TCR-CD3 complex, thymocytes fail to differentiate properly, underscoring its importance in T-cell development. Related references: PMID:10935641
• CD3 chains interact with CD4 and CD8 coreceptors, establishing a functional link that is required for activation and positive selection of CD4+ or CD8+ T cells.
• Subcellular localization is confined to the cell membrane, where CD3 functions as an integral membrane receptor complex.
• Key post-translational modifications include phosphorylation of ITAMs by Src family kinases LCK and FYN, as well as glycosylation and disulfide bond formation, which are critical for signal transduction and structural integrity.

Experimental Guidance and Technical Tips

• This antibody is supplied as a PE conjugate, allowing direct single-step staining for flow cytometry; titration experiments are recommended to determine the optimal concentration for your specific sample type.
• Validate the antibody in mouse lymphocyte populations (e.g., splenocytes, thymocytes) and use appropriate isotype controls to confirm specificity.
• Since the immunogen is a synthetic peptide derived from mouse CD3, the epitope may be linear and robust; however, fixable viability dyes or other fluorophore combinations should be tested for multicolor panels.
• Consider surface staining protocols and avoid permeabilization unless the epitope is preserved, as fixation/permeabilization can affect some epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to critical IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This PE anti-CD3 antibody supports T-cell immunophenotyping workflows, and our supply network covers rare targets and custom sourcing needs for murine immunology studies. Partner with CamelBio to accelerate your assay development with reliable, high-quality immunoreagents.