PE Rabbit anti-Rat IFN-gamma Monoclonal Antibody for Intracellular Flow Cytometry - P01581

Core Product Specifications and Parameters

Biological Background: IFN-gamma Function and Localization

• Interferon gamma (IFN-gamma) is a type II interferon cytokine mainly produced by activated T lymphocytes and natural killer (NK) cells, acting as a central mediator of innate and adaptive immunity.
• It exerts antimicrobial, antiviral, and antitumor activities by activating effector immune cells and enhancing antigen presentation via both MHC class I and II pathways.
• Signals through the JAK-STAT pathway: upon binding to its receptor IFNGR1, it induces activation of JAK2, JAK1, and STAT1, leading to STAT1 nuclear translocation and transcription of interferon-regulated genes such as IRF1.
• Upregulates MHC class I antigen processing by inducing replacement of constitutive proteasome subunits with immunoproteasome subunits and by inducing the PA28 activator, thereby enhancing peptide generation and repertoire for MHC loading.
• Promotes MHC class II expression by upregulating key molecules such as cathepsins B, H, and L (By similarity).
• Participates in hematopoietic stem cell regulation, affecting development, quiescence, and differentiation (By similarity).
• The protein is secreted and contains characteristic structural features, including a signal peptide, glycosylation sites, and a pyrrolidone carboxylic acid modification.
• The mature rat IFN-gamma protein has a predicted molecular weight of ~18 kDa (17918 Da), consistent with the immunogen design.

Experimental Guidance and Technical Tips

• The antibody is designed for intracellular flow cytometry (FC intra). Ensure appropriate fixation and permeabilization protocols are used to maintain PE fluorescence and antigen accessibility.
• Since the immunogen covers the mature peptide region (aa 20-156), the antibody likely recognizes the secreted, processed form of rat IFN-gamma.
• Perform titration experiments to determine the optimal working concentration for your specific cell type and stimulation conditions.
• Validate antibody performance with relevant positive controls, such as PMA/ionomycin-stimulated rat splenocytes or activated T-cell lines, and include isotype controls.
• Protect PE-conjugated samples from light to prevent photobleaching, and use appropriate compensation settings if combining with other fluorophores.

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CamelBio serves as a comprehensive one-stop bridge for IVD raw-material sourcing, connecting diagnostic manufacturers and research laboratories with high-quality reagents including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for rat interferon gamma targets and beyond. Our supply network covers rare target raw-material sourcing and offers technical support from concept to clinic, ensuring streamlined procurement for immunology and diagnostic development.