PE Rabbit anti-Rat CD8a Monoclonal Antibody for FC - P07725

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• CD8a is the alpha chain of the T-cell surface glycoprotein CD8, also known as the OX-8 membrane antigen or CD8 antigen 32 kDa chain. It is essential for adaptive immunity, serving as a coreceptor for MHC class I molecules on antigen-presenting cells.
• The CD8 coreceptor binds to the relatively invariant alpha-3 domain of MHC class I while the T-cell receptor (TCR) interacts with the peptide-presenting alpha-1 and alpha-2 domains, enabling T-cell recognition of cytosolic antigen-derived peptides.
• Upon co-engagement with the TCR-CD3 complex, CD8a recruits the Src kinase LCK to the cytoplasmic domain, initiating signaling cascades that promote lymphokine production, T-cell adhesion, motility, and cytotoxic activation against infected or transformed cells.
• CD8a homodimers expressed on natural killer (NK) cells provide a survival mechanism that permits conjugation and serial lysis of multiple target cells, while also supporting memory CD8+ T-cell differentiation and maintenance.
• Subcellular localization is predominantly at the cell membrane as a single-pass transmembrane glycoprotein, with the extracellular region containing an immunoglobulin-like domain.
• Post-translational modifications include N-linked glycosylation and palmitoylation at a cytoplasmic cysteine, which may influence membrane raft association and signaling.
• Tissue specificity: mainly expressed on cytotoxic T lymphocytes, NK cells, and dendritic cell subsets, with a molecular weight of approximately 32 kDa (glycosylated form) versus 26 kDa for the unmodified polypeptide.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fusion protein spanning amino acids 26-189 of rat CD8a (extracellular domain), which is predicted to generate antibodies recognizing the native, surface-exposed epitope on live cells.
• This PE-conjugated rabbit monoclonal antibody is designed for direct flow cytometric detection of CD8a-positive cells in rat samples; consider titrating the antibody to determine the optimal dilution for your sample type and background signal.
• For multicolor flow cytometry panels, ensure appropriate single-stain and fluorescence-minus-one (FMO) controls are included to accurately gate CD8a+ populations without spectral overlap artifacts.
• Since cross-reactivity is reported only for rat, validate reactivity in other species if required; alternative clones or host species may be needed for broader reactivity.
• For immunohistochemistry or other applications not listed, experimental validation is recommended as the antibody is qualified for FC only.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers, laboratories, and research institutions with validated antibodies, antigens, and ancillary reagents. This PE Rabbit anti-Rat CD8a monoclonal antibody supports flow cytometry-based immune phenotyping in rat models, ideal for development of immunology and oncology diagnostics. Through one-stop access to optimized monoclonal/polyclonal antibodies, bulk reagent supply, and rare target raw-material sourcing, CamelBio streamlines your journey from concept to clinic.