PE Rabbit anti-Rat CD25 Monoclonal Antibody for Flow Cytometry - P26897

Core Product Specifications and Parameters

Biological Background: CD25 (Interleukin-2 receptor subunit alpha) Function and Localization

• CD25, also known as Interleukin-2 receptor subunit alpha, IL-2Rα, or TAC antigen, is a type I membrane protein encoded by the Il2ra gene in rat (UniProt P26897).
• It serves as the alpha subunit of the high-affinity interleukin-2 (IL-2) receptor. Together with the beta (CD122) and gamma (CD132) subunits, it binds IL-2 and mediates signaling that is crucial for immune homeostasis.
• The receptor plays a key role in immune tolerance by controlling the activity of regulatory T cells (TREGs), which suppress the activation and expansion of autoreactive T cells.
• CD25 is constitutively expressed at high levels on CD4+Foxp3+ regulatory T cells and is transiently upregulated on activated effector T cells, making it an important marker for immunological studies.
• The extracellular region contains two Sushi (CCP/SCR) domains, which are characteristic of complement control proteins and are involved in ligand binding.
• Key structural features include an N-terminal signal peptide, multiple Sushi repeats, a single transmembrane helix, and a short cytoplasmic tail. The protein is modified by N-linked glycosylation and contains intrachain disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen for this monoclonal antibody comprises amino acids 22-235 of rat CD25, which corresponds to the entire extracellular domain. This design targets the native, membrane-distal region accessible in intact cells.
• As a PE-conjugated antibody, it is primarily intended for flow cytometry. We recommend initial titration using a relevant cellular model (e.g., activated rat splenocytes or T-cell lines) to identify the optimal staining concentration.
• Use an appropriate isotype control to discriminate positive staining from background. Proper Fc receptor blocking is advised when staining cells with high FcγR expression.
• Because CD25 expression can be sensitive to fixation and permeabilization, validate your sample preparation protocol to preserve surface epitopes if assessing cell surface markers.
• For intracellular detection of CD25, optimization of permeabilization steps is essential, as steric hindrance may alter antibody accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this PE-conjugated rabbit monoclonal antibody as a reliable raw material for IVD assay developers and research laboratories focusing on immune function and regulatory T-cell biology. Our portfolio includes validated antibody pairs, bulk ancillary reagents, and customized monoclonal/polyclonal antibodies to support seamless diagnostic development. For rare or specialized targets like CD25, CamelBio offers one-stop consulting and procurement to accelerate your R&D pipeline from concept to clinic.