PE Rabbit anti-Mouse/Rat CD31/PECAM1 mAb - Q08481

Core Product Specifications and Parameters

Biological Background: CD31/PECAM1 Function and Localization

• Platelet endothelial cell adhesion molecule (CD31/PECAM1) is a multifunctional glycoprotein encoded by the Pecam1 gene, with multiple isoforms produced by alternative splicing.
• It plays an essential role in leukocyte transendothelial migration (TEM) under inflammatory conditions, with Tyr-679 critical for TEM and recycling compartment dynamics.
• CD31 mediates homophilic endothelial cell–cell adhesion at cell junctions and heterophilic interaction with CD177 to facilitate neutrophil transmigration.
• It regulates macrophage-mediated phagocytosis: preventing phagocytosis of viable leukocytes via detachment signals, while promoting phagocytosis of apoptotic leukocytes.
• CD31 modulates bradykinin receptor BDKRB2 activation and regulates ERK1/2 signaling in response to bradykinin and hyperosmotic shock in endothelial cells.
• It is implicated in atherosclerosis susceptibility, as demonstrated by genetic studies. Related references: PMID:19048083
• Subcellularly, CD31 localizes to the cell membrane, membrane rafts, and cell junctions, consistent with its adhesion and signaling roles.
• Post‑translational modifications include glycosylation, palmitoylation, phosphorylation, and disulfide bond formation, contributing to its functional diversity.
• Tissue expression is prominent in lung and platelets, as confirmed at the protein level.

Experimental Guidance and Technical Tips

• The immunogen spans the N‑terminal extracellular region (aa 18‑220), which includes Ig‑like domains critical for homophilic interactions; this design is suitable for detecting surface CD31 by flow cytometry.
• Given confirmed cross‑reactivity with mouse and rat, this antibody is appropriate for studies in both species; however, species‑specific controls should be included.
• For flow cytometry, perform antibody titration to determine the optimal staining concentration in your sample system.
• This antibody is validated for flow cytometry; when considering other applications such as immunohistochemistry or western blotting, test compatibility under the required conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of validated IVD antibodies, including this anti‑CD31/PECAM1 monoclonal antibody, to support your research in vascular biology, inflammation, and atherosclerosis. Beyond single reagents, we provide matched antibody pairs, bulk ancillary reagents, and customized sourcing for rare‑target raw materials, bridging your project from concept to clinic.