PE Rabbit anti-Mouse/Rat CD27 Monoclonal Antibody for FC - P41272

Core Product Specifications and Parameters

Biological Background: CD27 Function and Localization

• CD27 (T-cell activation antigen CD27, TNFRSF7) is a single-pass type I transmembrane receptor belonging to the tumor necrosis factor receptor superfamily.
• It serves as a costimulatory immune-checkpoint receptor on T-cells, NK-cells, and B-cells; binding to its ligand CD70/CD27L on B-cells triggers the CD70-CD27 signaling pathway, mediating antigen-specific T-cell activation and expansion for B-cell immune surveillance. Related references: PMID:11062504
• CD70 ligation activates the TRAF2-PTPN6 axis, inhibiting LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory (By similarity).
• Activation on early progenitor cells delivers a negative feedback signal to leukocyte differentiation, thereby modulating hematopoiesis. Related references: PMID:15723067
• CD27 negatively regulates the function of Th2 lymphocytes in adipose tissue. Related references: PMID:38386557
• Subcellular localization: primarily on the cell membrane.
• Tissue expression: detected in thymus and spleen but not in non-lymphoid tissues; also expressed on myeloid progenitor cells. Related references: PMID:8384562 PMID:15723067
• Post-translational modifications: glycosylated protein with disulfide bonds, contributing to its structural stability.

Experimental Guidance and Technical Tips

• For flow cytometry, consider titrating the antibody to determine the optimal concentration and include a suitable isotype control.
• Because CD27 is expressed on T, B, and NK cells, ensure appropriate gating strategies and use viability dyes to exclude dead cells.
• The immunogen corresponds to the extracellular domain (aa 24–182), suggesting the antibody binds an extracellular epitope accessible on live cells.
• As a PE conjugate, protect the antibody from light and avoid freeze-thaw cycles.
• Cross-reactivity with rat has been reported; validate specificity in your experimental system if working with different strains.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to premium IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This PE-conjugated anti-CD27 antibody supports immunophenotyping and immune-checkpoint research, with additional rare-target sourcing and technical consulting available through our integrated supply network. Partner with CamelBio to accelerate your workflow from concept to clinic.