PE Rabbit anti-Mouse S100A9 Monoclonal Antibody for Intracellular Flow Cytometry - P31725

Core Product Specifications and Parameters

Biological Background: S100A9 Function and Localization

• S100A9 is a calcium- and zinc-binding protein that plays a pivotal role in regulating inflammatory processes and immune response, including neutrophil chemotaxis, phagocytosis, and degranulation.
  Related references: PMID:15331440, PMID:17767165, PMID:18403730
• Intracellularly, S100A9 facilitates leukocyte arachidonic acid trafficking and metabolism, modulates the tubulin-dependent cytoskeleton during phagocyte migration, and activates the neutrophilic NADPH-oxidase.
  Related references: PMID:15331440
• Extracellularly, S100A9 exerts pro-inflammatory, antimicrobial, oxidant-scavenging, and apoptosis-inducing activities.
  Related references: PMID:21382888
• S100A9 acts as an alarmin or damage-associated molecular pattern (DAMP), stimulating innate immune cells by binding to Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER).
  Related references: PMID:17767165, PMID:18403730, PMID:19402754
• Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways, amplifying the pro-inflammatory cascade.
  Related references: PMID:17767165, PMID:18403730, PMID:19402754
• Primarily exists as a calprotectin heterodimer (S100A8/S100A9), which integrates intracellular and extracellular immune functions.
• Exhibits antimicrobial activity against bacteria and fungi, likely via chelation of Zn²⁺ essential for microbial growth.
• S100A9 is predominantly secreted but can also be found in the cytoplasm, associated with the cytoskeleton, and on the cell membrane.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering the full-length mouse S100a9 (residues 1–113); thus the antibody is expected to recognize native S100A9 in intracellular flow cytometry. For optimal staining, perform fixation and permeabilization appropriate for nuclear/cytoplasmic proteins and titrate the PE-conjugated antibody to determine the optimal signal-to-noise ratio.
• S100A9 is highly expressed in myeloid lineage cells (neutrophils, monocytes, macrophages). Use relevant positive controls such as mouse bone marrow or splenocytes, and include isotype controls and Fc blocking to minimize non-specific binding.
• For intracellular flow cytometry, confirm antibody specificity using S100A9-knockout cells or blocking with the recombinant immunogen if available.
• As this is a PE-conjugated primary antibody, it enables direct single-step detection, reducing background from secondary antibodies and simplifying multicolor panels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials. This PE anti-mouse S100A9 monoclonal antibody supports immunology and inflammation research, with applications in flow cytometry. Beyond this product, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare or custom targets — helping you accelerate development from concept to clinic.