PE Rabbit anti-Mouse RANK/CD265 Monoclonal Antibody for Flow Cytometry - O35305

Core Product Specifications and Parameters

Biological Background: RANK/CD265 Function and Localization

• RANK (also known as ODFR, TRANCE-R) is a member of the tumor necrosis factor receptor superfamily (TNFRSF11A), encoded by the Tnfrsf11a gene in mouse. It is a transmembrane receptor.
• RANK serves as the receptor for RANKL (TNFSF11), and this interaction is essential for RANKL-mediated osteoclastogenesis. It promotes the differentiation of osteoclasts from hematopoietic precursors. Related references: PMID:20483727, PMID:23478294, PMID:9878548
• Binding of RANK to EEIG1 promotes osteoclastogenesis by facilitating NFATC1 transcription and PLCG2 activation. Related references: PMID:23478294
• RANK is involved in the regulation of interactions between T-cells and dendritic cells, highlighting its role beyond bone metabolism. Related references: PMID:9367155
• RANK is localized to the cell membrane and is enriched in membrane rafts, which are microdomains important for signal transduction.
• RANK has ubiquitous expression with high levels in trabecular bone, thymus, small intestine, lung, brain, and kidney, and weaker expression in spleen and bone marrow.
• RANK undergoes glycosylation and phosphorylation, and contains disulfide bonds, a signal peptide, and a transmembrane helix. It also binds sodium and metal ions, which may be relevant for its structure and function.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 31-213 of mouse RANK, corresponding to the extracellular domain. This design targets the ligand-binding region, making it suitable for applications detecting surface-expressed RANK.
• For flow cytometry, ensure proper isotype controls and titrate the antibody for optimal staining. When using PE conjugate, account for compensation settings in multicolor panels.
• Consider validating the antibody in relevant cell systems such as RANKL-stimulated RAW 264.7 or primary osteoclast precursors.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we offer a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents for bone metabolism and immunology research. Our one-stop sourcing service simplifies procurement from early-stage R&D to clinical applications, ensuring consistent supply of high-quality antibodies like this PE-conjugated anti-mouse RANK monoclonal antibody.