PE Rabbit anti-Mouse PD-L2/CD273/B7-DC Monoclonal Antibody for Flow Cytometry - Q9WUL5

Core Product Specifications and Parameters

Biological Background: PD-L2/CD273/B7-DC Function and Localization

• PD-L2 (Programmed cell death 1 ligand 2), also referred to as butyrophilin B7-DC (B7-DC) or CD273, is a transmembrane glycoprotein of the immunoglobulin superfamily, containing immunoglobulin-like domains and disulfide bonds.
• It plays a critical role in the induction and maintenance of immune tolerance to self, acting as a ligand for the inhibitory receptor PD-1 (PDCD1) on T cells, thereby inhibiting T-cell proliferation by blocking cell cycle progression and cytokine production. Related references: PMID:11224527 PMID:11283156 PMID:12719480
• The protein is localized to the cell membrane and possesses a single transmembrane helix (UniProt keyword).
• Tissue expression studies in mice show high levels in placenta and liver, with weaker expression in heart, spleen, lymph nodes, and thymus. It is also expressed in immature and mature bone marrow-derived dendritic cells and splenic dendritic cells, and some lymphoid-origin tumor cell lines.
• Post-translational modifications include glycosylation, and the protein contains a signal peptide, an immunoglobulin domain, and intramolecular disulfide bonds, critical for structural integrity and receptor binding.
• Keywords also indicate involvement in adaptive immunity and receptor activity, highlighting its role in immune checkpoint regulation.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fusion protein covering the extracellular domain (amino acids 20-123) of mouse PD-L2, which is expected to generate an antibody recognizing the native protein in flow cytometry applications.
• For flow cytometry, consider titrating the antibody to determine optimal staining concentration in the relevant cell type, such as mouse dendritic cells or PD-L2-transfected cell lines.
• Include appropriate isotype controls and viability dyes to ensure specificity and accurate gating.
• Validate the reactivity in your sample system, as cross-reactivity is only confirmed for mouse.

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