PE Rabbit anti-Mouse Ly-6A/E (Sca-1) Monoclonal Antibody for FC - P05533

Core Product Specifications and Parameters

Biological Background: Ly-6A/E (Sca-1) Function and Localization

• Ly-6A/E, also known as Stem cell antigen 1 (Sca-1) or T-cell activating protein (TAP), is a mouse glycosylphosphatidylinositol (GPI)-anchored cell surface protein encoded by the Ly6a gene.
• It functions as a co-stimulatory molecule in T-cell activation, contributing to immune response regulation.
• The protein is widely expressed across multiple tissues, consistent with its role in stem cell biology and immune surveillance.
• Ly-6A/E is anchored to the outer leaflet of the cell membrane via a GPI linkage and contains a signal peptide, disulfide bonds, and N-linked glycosylation sites, as indicated by UniProt keywords.
• Direct protein sequencing evidence confirms the mature protein, which has a predicted molecular weight of approximately 14.4 kDa (UniProt), though glycosylation and the GPI anchor may result in a higher apparent molecular weight on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen was designed using the extracellular domain (amino acids 27-111) of mouse Ly-6A/E, which eliminates the signal peptide and focuses the antibody response on the mature, surface-exposed region. For optimal recognition in flow cytometry, ensure cells are viable and non-permeabilized to preserve surface antigen integrity.
• This PE-conjugated monoclonal antibody is suitable for direct immunofluorescence staining in flow cytometry. Consider using appropriate isotype controls and Fc receptor blocking reagents to minimize non-specific binding.
• Protect the antibody conjugate from prolonged light exposure to maintain fluorophore stability. Validate the antibody in the relevant mouse strain or cell line system, as Ly-6A/E expression may vary among mouse haplotypes (e.g., Ly-6a vs. Ly-6b).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with high-quality IVD raw materials, offering a comprehensive portfolio that includes validated monoclonal antibodies, antibody pairs, and ancillary reagents. This Ly-6A/E (Sca-1) antibody supports stem cell and immunology research applications, and CamelBio can facilitate bulk orders, custom conjugations, and sourcing of rare-target raw materials to streamline your assay development pipeline. Our integrated supply chain and technical consultation help accelerate your path from concept to clinic.