PE Rabbit Anti-Mouse LAMP1/CD107a Monoclonal Antibody for FC - P11438

Core Product Specifications and Parameters

Biological Background: LAMP1/CD107a Function and Localization

• LAMP1 (Lysosome-associated membrane glycoprotein 1), also known as CD107a, LGP-120, LGP-A, P2B, and Lamp-1, is a heavily glycosylated transmembrane protein encoded by the Lamp1 gene in mice.
• It plays a critical role in lysosome biogenesis, pH regulation, autophagy, and cholesterol homeostasis by acting as a direct inhibitor of the proton channel TMEM175, thereby facilitating lysosomal acidification (PubMed:15121881). Related references: PMID:15121881
• In natural killer (NK) cells, LAMP1 is essential for cytotoxicity, mediating cytotoxic granule movement and perforin trafficking to the lytic granule, while also protecting NK cells from self-degranulation damage (PubMed:23847195). Related references: PMID:23847195
• It localizes to the membranes of lysosomes, late endosomes, and cytolytic granules, and can also be present on the cell surface upon degranulation.
• LAMP1 presents carbohydrate ligands to selectins, facilitating cell adhesion and migration.
• Its expression is implicated in tumor cell metastasis (PubMed:2676155). Related references: PMID:2676155
• Structurally, LAMP1 is a type I transmembrane protein with a large luminal domain, a single transmembrane helix, and a short cytoplasmic tail; it contains multiple disulfide bonds and N-glycosylation sites.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your cell type and assay conditions.
• Include appropriate isotype controls and fluorescence-minus-one (FMO) controls to set gates accurately.
• As LAMP1 is primarily intracellular (lysosomal), detection of surface LAMP1 (e.g., as a degranulation marker) may require stimulation; consider cell permeabilization if total LAMP1 is to be measured.
• The immunogen sequence (aa 208–363) lies within the large luminal domain; the antibody may be suitable for both native and denatured LAMP1, but performance in other applications (e.g., WB, IHC) has not been tested and requires validation.
• PE conjugation provides high brightness; ensure proper compensation when using multicolor panels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement solution for IVD raw materials, including this PE-conjugated anti-LAMP1 monoclonal antibody for immunology and cell biology applications. With access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, CamelBio supports your development from concept to clinic.