PE Rabbit anti-Mouse Integrin alpha 2/CD49b Monoclonal Antibody for FC - Q62469

Core Product Specifications and Parameters

Biological Background: Integrin alpha 2/CD49b Function and Localization

• Integrin alpha-2 (CD49b, GPIa, DX5) pairs with beta-1 subunit to form a collagen receptor, mediating platelet and cell adhesion to collagens.
• It functions in modulation of collagen and collagenase gene expression, force generation, and organization of newly synthesized extracellular matrix.
• Also acts as a receptor for laminins, collagen C-propeptides, and E-cadherin.
• Mice with a null mutation in Itga2 exhibit very early embryonic lethality, underscoring its essential role in development.
• The protein is a single-pass type I membrane glycoprotein, with a large extracellular domain containing integrin alpha repeats, and a short cytoplasmic tail.
• It binds divalent metal ions (calcium and magnesium) critical for ligand binding and integrin activation.
• Post-translational modifications include disulfide bonds and N-glycosylation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 168-366 within the extracellular domain, likely including regions involved in ligand interaction; confirm epitope accessibility in the target cell type and application.
• This antibody is directly conjugated to PE, making it suitable for flow cytometry without secondary reagents; titrate to determine optimal staining concentration (commonly 0.5–1 µg per 10^6 cells, adjusted empirically).
• For live-cell staining, ensure the antibody is used in an appropriate buffer containing protein to minimize nonspecific binding, and include appropriate isotype controls.
• Species reactivity is reported for mouse; verify cross-reactivity with other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This PE-conjugated anti-mouse Integrin alpha 2/CD49b antibody supports collagen receptor studies and platelet research. Our team also facilitates sourcing rare-target raw materials to accelerate your diagnostic development pipeline.