PE Rabbit anti-Mouse Integrin alpha 1/CD49a mAb for FC - Q3V3R4

Core Product Specifications and Parameters

Biological Background: Integrin alpha-1 Function and Localization

• Integrin alpha-1 (CD49a) is a type I transmembrane glycoprotein that pairs with integrin beta-1 to form the VLA-1 heterodimer, a receptor for laminin and collagen.
• It specifically recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen, a modification that enhances ligand binding.
• Integrin alpha-1/beta-1 mediates cell adhesion to extracellular matrix components and is implicated in anchorage-dependent, negative regulation of EGF-stimulated cell growth.
• The protein is localized to the plasma membrane (single-pass transmembrane) and possesses a large extracellular domain containing multiple repeats involved in ligand interaction and metal ion coordination.
• Key structural features include calcium and magnesium binding sites, disulfide bonds that stabilize the integrin alpha chain fold, and a cytoplasmic tail that transduces signals from the matrix.
• Gene: Itga1 in mouse; belongs to the integrin alpha chain family, sharing structural homology with other alpha subunits that pair with beta-1.
• Alternative names: VLA-1 (very late antigen-1), CD49A, laminin and collagen receptor.

Experimental Guidance and Technical Tips

• The antibody is supplied pre-conjugated with PE, enabling direct flow cytometry without the need for a secondary detection step.
• The immunogen spans the entire extracellular domain (aa 29–1141), favoring recognition of the native cell surface conformation.
• For optimal FC performance, titrate the antibody using relevant positive controls (e.g., activated mouse T cells) and include appropriate isotype controls.
• As a rabbit monoclonal, it provides high specificity and consistent lot-to-lot reproducibility; compensation settings should be optimized for PE in multicolor panels.
• The target protein migrates at ~130 kDa; ensure appropriate gating strategies when combining with other surface markers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this PE-conjugated anti-Integrin alpha 1/CD49a antibody as part of a broad IVD raw-material portfolio tailored for diagnostic manufacturers and research laboratories. From validated monoclonal/polyclonal antibodies and optimized antibody pairs to bulk ancillary reagents and rare-target sourcing, we provide end-to-end supply solutions for cell adhesion and extracellular matrix studies.