PE Rabbit anti-Mouse IL4 Monoclonal Antibody for Intracellular FC - P07750

Core Product Specifications and Parameters

Biological Background: Interleukin-4 Function and Localization

• Interleukin-4 (IL4) is a cytokine originally identified as B-cell stimulatory factor 1 (BSF-1) and B-cell growth factor 1, encoded by the Il4 gene.
• Secreted by mast cells, T-cells, eosinophils, and basophils, IL4 regulates antibody production, hematopoiesis, inflammation, and effector T-cell responses. Related references: PMID:3083412
• It induces class II MHC expression on resting B-cells and enhances both secretion and surface expression of IgE and IgG1. Related references: PMID:3498301
• IL4 stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and inducing RUFY4. Related references: PMID:26416964
• In the brain, IL4 contributes to memory and learning. Related references: PMID:25772794, PMID:28202615
• IL4 signals through two receptor complexes: type 1 (IL4R/IL2RG) on hematopoietic cells and type 2 (IL4R/IL13RA1) on nonhematopoietic cells, activating JAK1/JAK3 and STAT6. Related references: PMID:25847241, PMID:8624821
• The protein is secreted and annotated with keywords including autophagy, B-cell activation, cytokine, and glycoprotein.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 21–140 of mouse IL4, corresponding to the mature protein, ensuring recognition of the processed cytokine.
• For intracellular flow cytometry, consider optimizing fixation and permeabilization steps, and stimulate cells appropriately to induce IL4 expression.
• Include proper isotype controls and validate the antibody in your specific experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive one-stop access to IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents tailored for diagnostic manufacturers and research laboratories. This PE-labeled anti-mouse IL4 monoclonal antibody supports immune-oncology, inflammation, and neuroimmunology studies. Whether you need established catalog products or rare-target sourcing, CamelBio bridges your concept to clinic with reliable supply solutions.