PE Rabbit anti-Mouse IL1R1/CD121a Monoclonal Antibody for Flow Cytometry - P13504

Core Product Specifications and Parameters

Biological Background: Interleukin-1 Receptor Type 1 (IL1R1) Function and Localization

• Encodes the interleukin-1 receptor type 1 (IL1R1), also known as CD121a, a member of the immunoglobulin superfamily.
• Functions as a receptor for interleukin-1 alpha (IL1A), interleukin-1 beta (IL1B), and the interleukin-1 receptor antagonist (IL1RN).
• Upon ligand binding, IL1R1 associates with the co-receptor IL1RAP to form a high-affinity signaling complex that activates NF-kappa-B, MAPK, and other pathways.
• Signaling involves recruitment of adaptor molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 through the TIR domains.
• IL1RN antagonizes signaling by preventing association of IL1R1 with IL1RAP.
• Located at the cell membrane and can also be secreted; isoform 2 is expressed in various brain tissues.
• Undergoes post-translational modifications including glycosylation, disulfide bond formation, and phosphorylation.
• An alternative isoform cooperates with IL1RAP isoform 3 to mediate IL1B-induced neuronal activity, including potentiation of NMDA-induced calcium influx via Akt kinase activation.

Experimental Guidance and Technical Tips

• This antibody is PE-conjugated; ensure appropriate compensation controls and avoid light exposure during staining.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific experimental system.
• Validate the antibody in a relevant mouse cell line (e.g., macrophages or T cells) known to express IL1R1.
• The immunogen (amino acids 20–338) corresponds to the extracellular domain of mouse IL1R1, supporting detection of the receptor on the cell surface.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a one-stop sourcing bridge for IVD raw materials. This product targets IL1R1, a key mediator in inflammatory responses, supporting research in immunology and neuroinflammation. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and undertake rare target raw-material sourcing to streamline your development from concept to clinic.