PE Rabbit anti-Mouse IL-9 Monoclonal Antibody for Flow Cytometry (Intracellular) - P15247

Core Product Specifications and Parameters

Biological Background: IL-9 Function and Localization

• Interleukin-9 (IL-9), also known as cytokine P40 and T-cell growth factor P40, is a multifunctional cytokine encoded by the Il9 gene in mouse (UniProt P15247).
• IL-9 is primarily secreted by T-helper 2 (Th2) lymphocytes, mast cells, and NKT cells, and plays critical roles in immune responses against parasites, as well as in allergic inflammation. Related references: PMID:11070175, PMID:19433802
• It modulates intestinal epithelial permeability and contributes to adaptive immunity, particularly in mucosal tissues. Related references: PMID:12704113
• IL-9 promotes the differentiation of IL-17-producing helper T-cells (TH17) and stimulates the proliferation and differentiation of mast cells, linking it to inflammatory and allergic pathways. Related references: PMID:11070175, PMID:19433802
• Mechanistically, IL-9 signals through a receptor composed of the IL-9R alpha chain and the common gamma chain (IL2RG), leading to activation of JAK1 and JAK3 kinases and downstream STAT1, STAT3, and STAT5 transcription factors. STAT1 primarily drives differentiation genes, while STAT3 and STAT5 mediate anti-apoptotic effects. Related references: PMID:2145361, PMID:7718508, PMID:10464327
• The mature IL-9 protein is a secreted glycoprotein with a predicted molecular weight of approximately 16 kDa (UniProt: 16075 Da) and contains structural features typical of the four-helix bundle cytokine family.
• Subcellular localization is extracellular following signal peptide cleavage; it functions as a soluble mediator in the extracellular milieu.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 19–114 of mouse IL-9 (NP_032399.1), which encompasses the mature secreted protein lacking the signal peptide. This design is likely to generate antibodies recognizing the native, processed form of IL-9.
• As a PE-conjugated monoclonal antibody, it is optimized for intracellular flow cytometry (FC intra). For detection of intracellular IL-9, cells should be fixed and permeabilized using a suitable protocol prior to staining. A stimulation step (e.g., with PMA/ionomycin in the presence of a protein transport inhibitor) may be required to accumulate cytokine intracellularly in certain cell types.
• Since the antibody is reported to react with mouse IL-9, cross-reactivity with human or other species has not been evaluated; it is recommended to validate specificity in the intended sample system.
• The monoclonal rabbit origin may offer high affinity and specificity; however, titration is advisable to determine the optimal concentration for each experimental condition.
• Note that the antibody is shipped on ice bags; upon receipt, store as recommended (typically at 2–8°C in the dark for conjugated antibodies) to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with validated antibodies, antigen reagents, and ancillary products. This PE Rabbit anti-Mouse IL-9 mAb exemplifies our commitment to supplying high-quality immunological reagents for immune function studies. Beyond this product, CamelBio can assist in procuring validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and hard-to-find target raw materials to accelerate your assay development from concept to clinic.