PE Rabbit anti-Mouse IL-33R/ST2 mAb - P14719

Core Product Specifications and Parameters

Biological Background: IL-33R/ST2 Function and Localization

• IL-33R/ST2 is a receptor for interleukin-33 (IL-33), playing critical roles in innate and adaptive immunity, tissue homeostasis, and responses to environmental stresses. Related references: PMID:17675517, PMID:18450470, PMID:22660580
• Upon IL-33 binding and coreceptor IL1RAP association, it recruits MYD88, IRAK1, IRAK4, and TRAF6, leading to phosphorylation of MAP kinases (ERK1/2, p38, JNK) (By similarity).
• In tissue injury, it induces UCP2-dependent mitochondrial rewiring, attenuating ROS production and preserving Krebs cycle integrity for itaconate generation, driving GATA3-dependent differentiation of alternatively activated macrophages. Related references: PMID:34644537
• Alternatively spliced isoforms include a secreted soluble form that inhibits IL-33 signaling, acting as a decoy receptor.
• May be involved in helper T-cell function (By similarity).
• Predominantly expressed in hematopoietic tissues, macrophage, erythroid, epithelial, and fibroblast cell lines; isoform A in brain astrocytes/microglia, isoform B in brain endothelial cells.
• Subcellular localization includes the cell membrane and secreted forms; the protein contains signal peptide, transmembrane helix, and immunoglobulin-like domains.
• Post-translational modifications include glycosylation, phosphorylation, and ubiquitination, along with disulfide and isopeptide bond formation.

Experimental Guidance and Technical Tips

• The immunogen (aa 27-332) targets the extracellular region of mouse IL-33R/ST2, making this antibody appropriate for flow cytometry detection of surface-expressed receptor.
• As a PE-conjugated monoclonal antibody, it enables direct one-step staining; verify optimal dilution and signal-to-noise ratio in your specific cell model.
• Due to alternative splicing and the existence of a soluble isoform, the antibody may recognize both membrane-bound and shed ST2; confirm specificity in relevant sample types (e.g., hematopoietic cells or macrophage lines).
• Reported cross-reactivity with mouse; validate performance in target tissues or cell lines known to express IL-33R/ST2.
• For functional neutralization or blocking studies, consider that the epitope may lie within the IL-33 binding region; experimental validation is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. For IL-33/ST2-focused investigations, we supply validated antibody pairs, optimized monoclonal antibodies (including PE conjugates), bulk ancillary reagents, and can source rare-target biomaterials. Whether you need routine inventory or specialized custom reagents, CamelBio streamlines procurement to accelerate your assay development.