PE Rabbit anti-Mouse IL-22 Monoclonal Antibody for Intracellular Flow Cytometry - Q9JJY9

Core Product Specifications and Parameters

Biological Background: IL-22 Function and Localization

• Interleukin-22 (IL-22) is a member of the IL-10 cytokine family, also known as IL-10-related T-cell-derived-inducible factor (IL-TIF) or IL-22a.
• IL-22 critically modulates tissue responses during inflammation. Related references: PMID:33852830, PMID:35525330
• It is essential for the regeneration of epithelial cells, preserving barrier integrity after injury and limiting further tissue damage. Related references: PMID:33912578
• Unlike most cytokines, IL-22 signals through a heterodimeric receptor (IL22RA1/IL10RB) on non-immune cells, activating JAK1/TYK2-STAT3, ERK1/2, and PI3K/AKT pathways to promote cell survival and proliferation.
• IL-22 induces phosphorylation of GSK3B at Ser-9 and CTTN, contributing to epithelial cell spreading. Related references: PMID:24742671
• It is a secreted glycoprotein with intramolecular disulfide bonds, categorized as a reference proteome cytokine.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the mature sequence of mouse IL-22 (residues 34–179), which corresponds to the secreted form. This design is expected to elicit antibodies recognizing the native processed cytokine in biological samples.
• For intracellular flow cytometry, cells should be appropriately fixed and permeabilized to allow antibody access to cytoplasmic IL-22. A typical protocol includes stimulation (e.g., PMA/ionomycin) in the presence of a protein transport inhibitor, followed by fixation and permeabilization.
• Consider validating the staining pattern with relevant positive and negative controls, such as activated Th17 cells, to confirm specificity.
• The PE conjugate enables direct detection without a secondary antibody. According to the provided data, this antibody is specific for mouse IL-22 and does not cross-react with human IL-22.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw material procurement, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This PE-conjugated anti-mouse IL-22 antibody is designed to support flow cytometry-based studies in immunological and inflammatory disease research; we can assist with custom sourcing for rare target antibodies, conjugation services, and large-scale production needs to accelerate your diagnostic development.