PE Rabbit anti-Mouse IL-1β Monoclonal Antibody for Flow Cytometry (Intracellular) - P10749

Core Product Specifications and Parameters

Biological Background: IL-1β Function and Localization

• IL-1β is a potent pro-inflammatory cytokine initially discovered as the major endogenous pyrogen.
• It induces prostaglandin synthesis, neutrophil influx and activation, T-cell and B-cell activation, and fibroblast proliferation and collagen production.
• Promotes Th17 differentiation of T-cells and synergizes with IL-12 to stimulate IFNG synthesis from Th1 cells.
• Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
• Its mature form is released into the extracellular milieu through gasdermin-D pores following pyroptosis, propagating inflammation.
• Subcellularly localized in the cytoplasm, cytosol, lysosomes, and secreted via extracellular exosomes.
• Expressed primarily in activated macrophages at the protein level.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 118–269 of the mature mouse IL-1β protein, a region expected to be accessible in intracellular staining after appropriate fixation and permeabilization.
• For intracellular flow cytometry, optimize fixation/permeabilization protocols using positive controls such as LPS-stimulated mouse macrophages.
• The PE conjugate allows direct detection; consider proper compensation and isotype controls for accurate analysis.
• Validate antibody performance in relevant mouse sample systems where IL-1β is known to be induced (e.g., activated immune cells).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to reliable IVD raw materials and technical support. This PE-conjugated monoclonal antibody for mouse IL-1β facilitates high-sensitivity intracellular detection in flow cytometry, serving research in inflammation, innate immunity, and pyroptosis. As your sourcing partner, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare-target raw materials to accelerate your assay development from concept to clinic.