PE Rabbit anti-Mouse IL-17F Monoclonal Antibody - Q7TNI7

Core Product Specifications and Parameters

Biological Background: IL-17F Function and Localization

• Effector cytokine of innate and adaptive immunity; involved in antimicrobial host defense and maintenance of tissue integrity (PubMed:18025225, PubMed:19144317, PubMed:23255360). Related references: PMID:18025225, PMID:19144317, PMID:23255360
• Signals through IL17RA-IL17RC heterodimer or IL17RC homodimer, activating TRAF6-mediated NF-kappa-B and MAPK pathways to induce cytokines, chemokines, and antimicrobial peptides (PubMed:15477493, PubMed:17911633, PubMed:18025225, PubMed:28813677). Related references: PMID:15477493, PMID:17911633, PMID:18025225, PMID:28813677
• As a signature Th17 cytokine, drives neutrophil activation and recruitment to infection and inflammatory sites (PubMed:18025225). Related references: PMID:18025225
• Stimulates secretion of antimicrobial beta-defensins (DEFB1, DEFB103A, DEFB104A) by mucosal epithelial cells, restricting microbial entry (PubMed:19144317). Related references: PMID:19144317
• Regulates intestinal microbiota composition and immune tolerance by inducing antimicrobial proteins that control commensal Firmicutes and Bacteroidetes (PubMed:29915298). Related references: PMID:29915298
• Promotes sympathetic innervation of thermogenic adipose tissue via TGFB1 expression (PubMed:32076265). Related references: PMID:32076265
• Predominantly expressed by Th17 cells, intestinal epithelial cells, group 3 innate lymphoid cells, and diverse lamina propria immune cell subsets.

Experimental Guidance and Technical Tips

• This antibody is raised against recombinant mouse IL-17F (aa 1-161) and conjugated to PE, suitable for intracellular flow cytometry.
• For intracellular staining, optimize fixation and permeabilization protocols (e.g., using saponin-based buffers) according to your cell type.
• Validate staining in Th17-polarized mouse splenocytes or intestinal lamina propria lymphocytes, with appropriate isotype control.
• Confirm reactivity with recombinant mouse IL-17F protein or IL-17F-transfected cells as positive control.
• Note that IL-17F forms disulfide-linked homodimers and heterodimers with IL-17A; antibody specificity should be verified in the context of your experimental system.

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