PE Rabbit anti-Mouse IL-13 Monoclonal Antibody for Flow Cytometry (Intracellular) - P20109

Core Product Specifications and Parameters

Biological Background: IL-13 Function and Localization

• Cytokine that plays important roles in allergic inflammation and immune response to parasite infection (PubMed:15361238). Related references: PMID:15361238
• Synergizes with IL2 in regulating interferon-gamma synthesis and stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells.
• Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1 (PubMed:34789557). Related references: PMID:34789557
• Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF through a mechanism that partially involves suppression of NF-kappa-B.
• Functions on nonhematopoietic cells, including endothelial cells where it induces vascular cell adhesion protein 1/VCAM1, which is important in the recruitment of eosinophils.
• Exerts its biological effects through its receptors which comprises the IL4R chain and the IL13RA1 chain, to activate JAK1 and TYK2, leading to the activation of STAT6 (PubMed:34795444, PubMed:8871614). Related references: PMID:34795444, PMID:8871614
• IL13RA2 acts as a high affinity decoy for IL13 and mediates internalization and depletion of extracellular IL13 (PubMed:29305434). Related references: PMID:29305434
• Induces inflammatory response in esophageal epithelium which results in a dysregulation of desmosome intercellular adhesion junctions, via down-regulation of DSG1 (PubMed:24220297). Related references: PMID:24220297
• Secreted glycoprotein with disulfide bonds (UniProt keywords: Secreted, Signal, Disulfide bond, Glycoprotein).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20–131, representing the mature secreted form of IL-13 after signal peptide cleavage; verify specificity in the relevant sample system.
• For intracellular flow cytometry, consider an appropriate fixation/permeabilization protocol and include controls for proper detection of intracellular cytokines.
• PE conjugation may require attention to fluorophore compensation and photo-stability; titrate antibody concentration to optimize signal-to-noise ratio.
• Validate expression under stimulatory conditions known to induce IL-13 production, such as activated Th2 cells or mast cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. Our IL-13 antibody portfolio supports research in allergic inflammation, asthma, and cytokine signaling. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development.