PE Rabbit anti-Mouse IL-10RA/CD210 mAb for FC - Q61727

Core Product Specifications and Parameters

Biological Background: IL-10RA/CD210 Function and Localization

• Interleukin-10 receptor subunit alpha (IL-10RA, CDw210a, IL-10R1) is a cell surface receptor for the cytokine IL-10, playing a key role in limiting excessive tissue disruption caused by inflammation.
• Upon IL-10 binding, IL-10RA recruits IL-10RB to form a heterotetrameric complex, which activates associated JAK1 and TYK2 kinases, leading to phosphorylation of specific tyrosine residues on IL-10RA and subsequent STAT3 recruitment and phosphorylation (PubMed:8910398). Related references: PMID:8910398
• Phosphorylated STAT3 homodimerizes and translocates to the nucleus to induce anti-inflammatory gene expression.
• IL-10RA-mediated activation of STAT3 also inhibits starvation-induced autophagy (By similarity).
• The protein is localized at the cell membrane and in the cytoplasm.
• Post-translational modifications include disulfide bond formation, glycosylation, phosphorylation, and ubiquitination, reflecting its nature as a transmembrane glycoprotein and phosphoprotein.
• The predicted molecular weight is approximately 64 kDa, consistent with its migration in membrane protein analyses.

Experimental Guidance and Technical Tips

• The recombinant immunogen (aa 17-241) covers the extracellular domain, making this antibody potentially suitable for detecting native cell surface IL-10RA in flow cytometry without permeabilization.
• As a PE-conjugated antibody, it enables direct detection; remember to include an appropriate PE-conjugated isotype control in your staining panel.
• Titrate the antibody on your target cell type to determine the optimal concentration, and validate results in mouse samples where cross-reactivity has been confirmed.
• Since cross-reactivity is reported only for mouse, this reagent is not recommended for other species without prior validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies your supply chain with one-stop access to validated IVD raw materials, including monoclonal antibodies like this anti-IL-10RA for anti-inflammatory and cytokine signaling research. We offer optimized antibody pairs, bulk ancillary reagents, and specialized sourcing for rare targets, supporting your assay development from concept to clinic.