PE Rabbit anti-Mouse IgG2b mAb for FC - P01867-2

Core Product Specifications and Parameters

Biological Background: IgG2b Function and Localization

• The product targets Immunoglobulin heavy constant gamma 2B (Ig gamma-2B chain C region), isoform 2 of mouse IgG2b constant region (UniProt: P01867-2), encoded by the Ighg2b gene (GeneID: 16016).
• This protein is secreted and serves as the constant region of the IgG2b antibody heavy chain.
• The calculated molecular weight of the isoform is approximately 36.6 kDa (UniProt P01867-2).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 14-113 of mouse IgG2b isoform 2 (P01867-2), which targets a region within the constant domain; consider verifying the epitope specificity via peptide competition assays if needed.
• This antibody is provided as a PE conjugate and is validated for flow cytometry; ensure proper compensation and use isotype controls in FC panels.
• For optimal staining, titrate the antibody in the relevant mouse sample system and follow standard protocols for cell surface staining.
• Shipping condition is ice bag; handle with care to avoid freeze-thaw cycles that may affect conjugate stability.

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