PE Rabbit anti-Mouse IgG1 Monoclonal Antibody for FC - P01868

Core Product Specifications and Parameters

Biological Background: IgG1 Function and Localization

• The Ig gamma-1 chain C region (IgG1 constant region) is a major component of mouse immunoglobulin G1 antibodies.
• Encoded by the Ighg1 gene (also designated Igh-4), this chain is part of the immunoglobulin heavy chain family.
• Contains an immunoglobulin-like domain that mediates antigen binding and effector functions.
• Undergoes post-translational modifications including glycosylation (glycoprotein) and formation of disulfide bonds for structural stability.
• Localizes to the extracellular space and is secreted into serum (SUBCELLULAR LOCATION: Secreted).
• Alternative splicing generates distinct isoforms, including secreted and membrane-bound forms, the latter serving as the B cell receptor.
• Classified under keywords: 3D-structure, Alternative splicing, Direct protein sequencing, Disulfide bond, Glycoprotein, Immunoglobulin domain, Reference proteome, Secreted.
• Predicted molecular weight is approximately 35.7 kDa, consistent with the 36 kDa band observed in laboratory testing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 98–324 of the mouse IgG1 constant region, providing specificity for the Fc portion of the heavy chain.
• For flow cytometry (FC) applications, titrate the antibody to determine the optimal staining concentration in your model system.
• Use appropriate isotype controls and compensation controls when multiplexing with other fluorophores.
• Avoid prolonged exposure to light to preserve PE fluorescence intensity.
• Validate performance in mouse samples under your specific experimental conditions before applying to critical assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this PE Rabbit anti-Mouse IgG1 monoclonal antibody to support flow cytometry-based immunoglobulin profiling and B cell research. As a one-stop IVD raw-material partner, we offer validated monoclonal antibodies, optimized antibody pairs, bulk ancillary reagents, and assistance in sourcing rare targets—streamlining your path from concept to clinical application.