PE Rabbit anti-Mouse ICOS/CD278 Monoclonal Antibody for FC - Q9WVS0

Core Product Specifications and Parameters

Biological Background: ICOS/CD278 Function and Localization

• Inducible T-cell costimulator (ICOS), also known as CD278, AILIM, CCLP, or CRP-1, is a 22 kDa transmembrane receptor of the CD28 immunoglobulin superfamily.
• Expressed on activated T-cells and resting memory T-cells; high expression in thymic medulla, lymph node germinal centers, T-cell zones, and Peyer’s patches; low in spleen.
• Localized to the cell membrane as a single-pass type I transmembrane protein.
• Delivers costimulatory signals to T-cells upon binding to ICOSL on antigen-presenting cells, enhancing proliferation, cytokine secretion (e.g., IL-10), and expression of adhesion molecules. Related references: PMID:11343123, PMID:10657606
• Critical for differentiation of T follicular regulatory cells during immune challenges like viral infection. Related references: PMID:36754569
• Augments TCR-induced calcium flux via PLCG1 and actin remodeling, and independently activates the PI3K pathway. Related references: PMID:27693916, PMID:19915142
• Essential for T-cell–B-cell interaction, normal antibody responses to T-dependent antigens, and CD40-mediated immunoglobulin isotype switching. Related references: PMID:11343122
• Structurally, ICOS contains an extracellular immunoglobulin-like domain, a transmembrane helix, a cytoplasmic tail, and is modified by N-linked glycosylation and disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 21–142) of mouse ICOS, ensuring the antibody recognizes the native conformation; suitable for detecting surface ICOS by flow cytometry.
• For flow cytometry, titrate the antibody to determine optimal concentration for your cell type; use appropriate isotype controls.
• The PE conjugate allows direct detection without secondary antibodies; avoid repeated freeze-thaw cycles and protect from light.
• Validate the antibody in the relevant mouse tissue or cell line expressing ICOS (e.g., activated T-cells, thymus, lymph nodes).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to IVD raw materials, offering validated antibody pairs, bulk ancillary reagents, and rare target sourcing. For ICOS-related immune profiling, we supply optimized monoclonal antibodies and technical support to accelerate your assay development. Partner with us for reliable, one-stop procurement from concept to clinic.