PE Rabbit anti-Mouse I-A Monoclonal Antibody for FC - P14483

Core Product Specifications and Parameters

Biological Background: H-2 class II histocompatibility antigen, A beta chain Function and Localization

• The target protein is the H-2 class II histocompatibility antigen, A beta chain (also known as I-A, MHC II, Ia antigen, H-2I), encoded by the H2-Ab1 gene in mouse.
• It is a type I membrane glycoprotein with a signal peptide and a transmembrane helix, localizing to the cell surface (UniProt: SUBCELLULAR LOCATION).
• As a core component of MHC class II molecules, it plays a pivotal role in adaptive immunity by presenting exogenous peptides to CD4+ T cells.
• Key structural features include a disulfide bond and potential Ubl conjugation (ubiquitination) sites, contributing to its stability and regulation.
• The mature protein has a molecular weight of approximately 30 kDa (30,128 Da according to UniProt).
• Mouse H2-Ab1 products are widely used to study antigen presentation, immune responses, and autoimmune disease models.

Experimental Guidance and Technical Tips

• For flow cytometric staining, titrate the PE-conjugated antibody to determine the optimal concentration for your cell population and minimize background.
• Use appropriate single-stain compensation controls, especially if combining with other PE-labeled antibodies or in multicolor panels.
• Verify staining specificity with mouse cells expressing the relevant I-A and I-E allotypes; consider using Fc receptor blocking reagents to reduce non-specific binding.
• Protect the PE conjugate from prolonged light exposure to prevent photobleaching, and perform data acquisition promptly after staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with high-quality IVD raw materials. This PE-labeled anti-Mouse I-A monoclonal antibody is part of our comprehensive portfolio for immunology and MHC research. Beyond antibodies, we offer validated antibody pairs, bulk ancillary reagents, and specialized sourcing for rare targets to streamline your assay development from concept to clinic.