PE Rabbit anti-Mouse E-Cadherin/CD324 Monoclonal Antibody for FC - P09803

Core Product Specifications and Parameters

Biological Background: E-Cadherin/CD324 Function and Localization

• E-Cadherin/CD324 is a calcium-dependent cell adhesion protein that preferentially interacts homophilically to connect cells, contributing to sorting of heterogeneous cell types and regulating cell-cell adhesions, mobility, and proliferation of epithelial cells. Related references: PMID:11976333
• It promotes organization of radial actin fiber structure and cellular response to contractile forces via interaction with AMOTL2, and facilitates desmosome formation by recruiting DSG2 and DSP.
• The cleaved fragment E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors, inhibiting APP C99 and C83 production.
• Does not function as a receptor for L. monocytogenes internalin A (InlA); a single surface-exposed residue mutation can confer receptor activity.
• Subcellularly localized to adherens junctions, cell membrane, endosomes, trans-Golgi network, cytoplasm, and desmosomes.
• Expressed in non-neural epithelial tissues, including keratinocytes, organ of Corti cells, and granuloma macrophages.
• Post-translational modifications include disulfide bonds, glycosylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• For flow cytometry, consider titrating the antibody to determine the optimal staining concentration for your specific experimental conditions.
• PE is a bright fluorophore; ensure proper compensation when using in multi-color panels.
• Fixable viability dyes are recommended to exclude dead cells and improve data quality.
• This antibody is validated for mouse E-Cadherin; cross-reactivity with other species has not been tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, connecting diagnostic manufacturers and labs with high-quality reagents. This PE-conjugated anti-mouse E-Cadherin antibody supports research in cell adhesion, epithelial biology, and cancer, and it exemplifies our capability to supply validated monoclonal antibodies, bulk ancillary reagents, and rare-target raw materials for your diagnostic development needs.