PE Rabbit anti-Mouse CD90.2 Monoclonal Antibody for Flow Cytometry - P01831

Core Product Specifications and Parameters

Biological Background: CD90.2 (Thy-1) Function and Localization

• CD90.2, also known as Thy-1 membrane glycoprotein, is a GPI-anchored cell surface protein belonging to the immunoglobulin superfamily.
• Encoded by the Thy1 gene in mouse (Gene ID: 21838), it shares structural and functional homology with human THY1 (Gene ID: 7070).
• The primary function of Thy-1 involves mediating cell-cell and cell-matrix interactions critical for synaptogenesis and other events in the brain.
• The protein is localized to the outer leaflet of the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor.
• Post-translational modifications include N-linked glycosylation, intrachain disulfide bonds, and N-terminal pyrrolidone carboxylic acid formation.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody on mouse thymocytes or splenocytes to determine the optimal working dilution.
• Use appropriate isotype controls and fluorescence compensation controls to ensure accurate gating and minimal spillover.
• Protect the PE-conjugated antibody from prolonged light exposure and avoid repeated freeze-thaw cycles to maintain conjugate stability.
• Consider validating the antibody specificity in your specific experimental system using Thy-1 knockout or knockdown models if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated sourcing for IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents tailored for flow cytometry applications. With expertise in immune cell surface markers like CD90.2, our supply solutions support diagnostic development and research into cell-cell interactions and neurobiology. Contact us for bulk quantities, customized conjugations, and rare-target material procurement.