PE Rabbit anti-Mouse CD8a Monoclonal Antibody for Flow Cytometry - P01731

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• CD8a, also known as Ly-2 or Lyt-2, is a T-cell surface glycoprotein that serves as a coreceptor for MHC class I molecules.
• It plays an essential role in the adaptive immune response by interacting simultaneously with the T-cell receptor (TCR) and MHC class I:peptide complexes on antigen-presenting cells.
• CD8a recruits the Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling cascades that lead to T-cell activation, lymphokine production, and cytotoxic T-lymphocyte (CTL) function.
• In CTLs, this mechanism enables recognition and elimination of infected or tumor cells, while in NK cells, CD8A homodimers promote survival and serial killing of target cells.
• The protein is localized at the cell membrane as a type I transmembrane glycoprotein and contains an extracellular immunoglobulin-like domain.
• Post-translational modifications include disulfide bonds, glycosylation, and palmitoylation, which contribute to its stability and membrane association.
• Alternative splicing generates multiple isoforms, and CD8a expression is a hallmark of cytotoxic T cells and a subset of NK cells, also playing a role in memory CD8 T-cell differentiation.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant protein covering amino acids 28–196 of mouse CD8a, representing the extracellular domain, which ensures recognition of the native cell-surface antigen.
• This PE-conjugated monoclonal antibody is designed for flow cytometry (FC); consider titrating the antibody to optimal concentrations for your specific cell type and panel.
• Since CD8a is expressed on cytotoxic T cells and certain NK cells, appropriate gating strategies and viability staining are recommended to obtain clear population resolution.
• Validate the antibody in your relevant sample system (e.g., mouse splenocytes or tumor-infiltrating lymphocytes) and include isotype controls to confirm specificity.
• For multicolor flow panels, check potential spectral overlap of PE with other fluorophores and use appropriate compensation settings.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with one-stop access to high-quality IVD raw materials. For this mouse CD8a antibody, we can supply bulk orders and offer technical consulting to accelerate your T-cell biology and immuno-oncology programs.