PE Rabbit anti-Mouse CD86 mAb - P42082

Core Product Specifications and Parameters

Biological Background: T-lymphocyte activation antigen CD86 Function and Localization

• CD86 (also known as B7-2, ETC-1, Ly-58, and CD28 ligand 2) is a member of the immunoglobulin superfamily and a key costimulatory molecule.
• Functions as a ligand for the CD28 receptor, providing an essential secondary signal that augments MHC/TCR-mediated activation of naive T-cells.
• Acts as an inhibitor of T-cell activation by binding to CTLA4, a decoy receptor that blocks CD28-mediated T-cell priming. Related references: PMID:7504059, PMID:7513726
• Regulates B-cell function by controlling IgG1 production and activating the NF-kappa-B pathway upon CD40 engagement. Related references: PMID:23241883
• Localizes to the cell membrane and is expressed on the surface of antigen-presenting cells.
• Post-translational modifications include glycosylation and disulfide bond formation; also undergoes ubiquitination.
• Alternative splicing produces multiple isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 24–245), ensuring recognition of the native conformation for flow cytometry.
• For flow cytometry, titrate the PE-conjugated antibody to determine the optimal staining concentration and include an Fc-blocking step to reduce non-specific binding.
• Use appropriate viability dyes and gates to exclude dead cells during acquisition.
• Validate staining in the relevant sample system (e.g., mouse splenocytes or antigen-presenting cell lines) and include isotype controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions streamlined access to validated IVD raw materials, including antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This PE-conjugated anti-mouse CD86 antibody supports T-cell costimulation research, and our team can assist with sourcing rare-target raw materials and complementary reagents to accelerate your assay development workflow.