PE Rabbit anti-Mouse CD83 Monoclonal Antibody for Flow Cytometry - O88324

Core Product Specifications and Parameters

Biological Background: CD83 Function and Localization

• CD83 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily, characterized by an immunoglobulin domain, disulfide bonds, glycosylation sites, and a transmembrane helix.
• It is predominantly expressed on the surface of dendritic cells and lymphocytes, functioning as a key modulator of immune responses.
• Plays an essential role in CD4+ T-cell selection, differentiation, and stability by regulating the activity of the E3 ubiquitin ligase MARCHF8, which controls MHC II trafficking. Related references: PMID:27503071, PMID:27503069
• Additionally, CD83 inhibits MARCHF1 association with MHC II and MHC II ubiquitination, influencing antigen presentation (By similarity).
• Acts as an important modulator of protective responses against acute infections. Related references: PMID:31527313
• Subcellular localization is primarily at the membrane.
• Abundantly expressed in spleen and brain, but detectable in most tissues.
• Keywords from UniProt include signal peptide, immunoglobulin domain, glycoprotein, disulfide bond, and transmembrane regions, consistent with its role in immune regulation.

Experimental Guidance and Technical Tips

• This PE-conjugated rabbit monoclonal antibody can be used directly for flow cytometry detection of mouse CD83 without the need for a secondary antibody.
• Consider including appropriate isotype controls to assess non‑specific binding in your flow cytometry experiments.
• Based on tissue specificity, spleen- or brain-derived cells may yield stronger CD83 signals; for tissues with lower expression, optimize antibody titration.
• Prepare single‑cell suspensions from lymphoid organs (e.g., spleen, lymph nodes) or other target tissues to ensure reliable staining.
• Validate antibody performance in the specific sample type and experimental conditions, as expression levels may vary with cell activation or differentiation status.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with simplified access to IVD raw materials, technical support, and project consulting—from early development to market readiness. For CD83-focused studies in immunology or immune‑oncology, we offer validated monoclonal antibodies, optimized antibody pairs, bulk ancillary reagents, and assistance in sourcing rare‑target raw materials. Contact us to discuss how we can support your next project with reliable supply solutions.