PE Rabbit anti-Mouse CD82 Monoclonal Antibody for Flow Cytometry - P40237

Core Product Specifications and Parameters

Biological Background: CD82 Function and Localization

• CD82, also known as Kai1, Tspan27, IA4, C33 antigen, and inducible membrane protein R2, is a member of the tetraspanin superfamily. It is a structural component of tetraspanin-enriched microdomains (TERMs), which serve as platforms for receptor clustering and signaling.
• Core functions include attenuation of EGF signaling by facilitating ligand-induced endocytosis and desensitization of EGFR, mediated through ubiquitination and trafficking of EGFR via PKC-dependent CBL phosphorylation. It also modulates cell adhesion and migration by regulating integrins such as alpha4/ITA4, alpha6/ITGA6, and beta1/ITGB1, and interferes with urokinase-type plasminogen activator (PLAU) and its receptor (PLAUR) to decrease plasminogen activation.
• In immune cells, CD82 associates with CD4 or CD8 to deliver costimulatory signals for the TCR/CD3 pathway and plays a role in restraining phagocyte migration while supporting macrophage activation. It is recruited to pathogen-containing phagosomes prior to lysosomal fusion and participates in antigen presentation independently of Toll-like receptor signaling. Related references: PMID:21149584
• CD82 restrains phagocyte migration but supports macrophage activation and the acquisition of anti-inflammatory phenotypes. Related references: PMID:35754722
• It controls TLR9 trafficking to acidified CpG-containing compartments by regulating the interaction between TLR9 and VAMP3 and subsequent myddosome assembly, and it inhibits LPS-induced inflammatory responses by preventing LPS binding to TLR4. Related references: PMID:31408613
• In angiogenesis, CD82 acts to switch the angiogenic milieu to a quiescent state by binding and sequestering VEGFA and PDGFA, thereby inhibiting signaling through their respective cell surface receptors. Related references: PMID:34530889
• Subcellular localization includes the cell membrane, cytoplasmic vesicles, and phagosomes. Tissue expression is highest in the spleen and kidney, with low levels in skeletal muscle and heart.
• The protein is post-translationally modified by palmitoylation and glycosylation, with a predicted molecular weight of approximately 30 kDa (consistent with the observed 30kDa).

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1–266 of mouse CD82, encompassing the full extracellular and transmembrane domains, which supports recognition of native epitopes for flow cytometry applications.
• As a PE-conjugated antibody, it is ready-to-use without a secondary detection reagent; consider titrating the optimal dilution for your specific cell type and experimental conditions.
• Include appropriate isotype controls and, if analyzing intracellular targets or phosphorylated proteins, validate fixation and permeabilization protocols in your system.
• For mouse samples with high Fc receptor expression, consider using an Fc block to minimize non-specific binding.
• The reported cross-reactivity with mouse suggests suitability for murine models; verify reactivity in other species as needed.
• Performance may vary with cell preparation methods; consider validating with known positive and negative cell populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with end-to-end access to IVD raw materials, bridging the gap from concept to clinic. Our portfolio includes this anti-mouse CD82 antibody to support immunology, inflammation, and cancer research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development and manufacturing workflows.